LOCUS       sequence-280829-        3146 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1953
FEATURES             Location/Qualifiers
     source          1..3146
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagtc ttcggataag ctctatcgag tcgagtacgc caagagcggg
       61 cgcgcctctt gcaagaaatg cagcgagagc atccccaagg actcgctccg gatggccatc
      121 atggtgcagt cgcccatgtt tgatggaaaa gtcccacact ggtaccactt ctcctgcttc
      181 tggaaggtgg gccactccat ccggcaccct gacgttgagg tggatgggtt ctctgagctt
      241 cggtgggatg accagcagaa agtcaagaag acagcggaag ctggaggagt gacaggcaaa
      301 ggccaggatg gaattggtag caaggcagag aagactctgg gtgactttgc agcagagtat
      361 gccaagtcca acagaagtac gtgcaagggg tgtatggaga agatagaaaa gggccaggtg
      421 cgcctgtcca agaagatggt ggacccggag aagccacagc taggcatgat tgaccgctgg
      481 taccatccag gctgctttgt caagaacagg gaggagctgg gtttccggcc cgagtacagt
      541 gcgagtcagc tcaagggctt cagcctcctt gctacagagg ataaagaagc cctgaagaag
      601 cagctcccag gagtcaagag tgaaggaaag agaaaaggcg atgaggtgga tggagtggat
      661 gaagtggcga agaagaaatc taaaaaagaa aaagacaagg atagtaagct tgaaaaagcc
      721 ctaaaggctc agaacgacct gatctggaac atcaaggacg agctaaagaa agtgtgttca
      781 actaatgacc tgaaggagct actcatcttc aacaagcagc aagtgccttc tggggagtcg
      841 gcgatcttgg accgagtagc tgatggcatg gtgttcggtg ccctccttcc ctgcgaggaa
      901 tgctcgggtc agctggtctt caagagcgat gcctattact gcactgggga cgtcactgcc
      961 tggaccaagt gtatggtcaa gacacagaca cccaaccgga aggagtgggt aaccccaaag
     1021 gaattccgag aaatctctta cctcaagaaa ttgaaggtta aaaaacagga ccgtatattc
     1081 cccccagaaa ccagcgcctc cgtggcggcc acgcctccgc cctccacagc ctcggctcct
     1141 gctgctgtga actcctctgc ttcagcagat aagccattat ccaacatgaa gatcctgact
     1201 ctcgggaagc tgtcccggaa caaggatgaa gtgaaggcca tgattgagaa actcgggggg
     1261 aagttgacgg ggacggccaa caaggcttcc ctgtgcatca gcaccaaaaa ggaggtggaa
     1321 aagatgaata agaagatgga ggaagtaaag gaagccaaca tccgagttgt gtctgaggac
     1381 ttcctccagg acgtctccgc ctccaccaag agccttcagg agttgttctt agcgcacatc
     1441 ttgtcccctt ggggggcaga ggtgaaggca gagcctgttg aagttgtggc cccaagaggg
     1501 aagtcagggg ctgcgctctc caaaaaaagc aagggccagg tcaaggagga aggtatcaac
     1561 aaatctgaaa agagaatgaa attaactctt aaaggaggag cagctgtgga tcctgattct
     1621 ggactggaac actctgcgca tgtcctggag aaaggtggga aggtcttcag tgccaccctt
     1681 ggcctggtgg acatcgttaa aggaaccaac tcctactaca agctgcagct tctggaggac
     1741 gacaaggaaa acaggtattg gatattcagg tcctggggcc gtgtgggtac ggtgatcggt
     1801 agcaacaaac tggaacagat gccgtccaag gaggatgcca ttgagcactt catgaaatta
     1861 tatgaagaaa aaaccgggaa cgcttggcac tccaaaaatt tcacgaagta tcccaaaaag
     1921 ttctaccccc tggagattga ctatggccag gatgaagagg cagtgaagaa gctgacagta
     1981 aatcctggca ccaagtccaa gctccccaag ccagttcagg acctcatcaa gatgatcttt
     2041 gatgtggaaa gtatgaagaa agccatggtg gagtatgaga tcgaccttca gaagatgccc
     2101 ttggggaagc tgagcaaaag gcagatccag gccgcatact ccatcctcag tgaggtccag
     2161 caggcggtgt ctcagggcag cagcgactct cagatcctgg atctctcaaa tcgcttttac
     2221 accctgatcc cccacgactt tgggatgaag aagcctccgc tcctgaacaa tgcagacagt
     2281 gtgcaggcca aggtggaaat gcttgacaac ctgctggaca tcgaggtggc ctacagtctg
     2341 ctcaggggag ggtctgatga tagcagcaag gatcccatcg atgtcaacta tgagaagctc
     2401 aaaactgaca ttaaggtggt tgacagagat tctgaagaag ccgagatcat caggaagtat
     2461 gttaagaaca ctcatgcaac cacacacaat gcgtatgact tggaagtcat cgatatcttt
     2521 aagatagagc gtgaaggcga atgccagcgt tacaagccct ttaagcagct tcataaccga
     2581 agattgctgt ggcacgggtc caggaccacc aactttgctg ggatcctgtc ccagggtctt
     2641 cggatagccc cgcctgaagc gcccgtgaca ggctacatgt ttggtaaagg gatctatttc
     2701 gctgacatgg tctccaagag tgccaactac tgccatacgt ctcagggaga cccaataggc
     2761 ttaatcctgt tgggagaagt tgcccttgga aacatgtatg aactgaagca cgcttcacat
     2821 atcagcaagt tacccaaggg caagcacagt gtcaaaggtt tgggcaaaac tacccctgat
     2881 ccttcagcta acattagtct ggatggtgta gacgttcctc ttgggaccgg gatttcatct
     2941 ggtgtgaatg acacctctct actatataac gagtacattg tctatgatat tgctcaggta
     3001 aatctgaagt atctgctgaa actgaaattc aattttaaga cctccctgtg gtagtaatga
     3061 actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg tggaaaggac
     3121 gagagacggc gttgtgaaag gaagag
//
