LOCUS       sequence-280835-        2090 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1961
FEATURES             Location/Qualifiers
     source          1..2090
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccaa tgtgggatcg atgtttcaat attggaagcg ctttgattta
       61 cagcagctgc agagagaact cgatgccacc gcaacggtat tggcgaaccg gcaggatgaa
      121 agtgagcagt ccagaaagcg gcttatcgaa cagagccggg agttcaagaa gaacactcca
      181 gagattgatg cactgagtaa aagaagcaag gaagctgaag cagctttctt gaatgtctac
      241 aaaagattga ttgacgtccc agatcccgta ccagctttgg atctcggaca gcaactccag
      301 ctcaaagtgc agcgcctgca cgatattgaa acagagaacc agaaacttag ggaaactctg
      361 gaagaataca acaaggaatt tgctgaagtg aaaaatcaag aggttacgat aaaagcactt
      421 aaagagaaaa tccgagaata tgaacagaca ctgaagaacc aagccgaaac catagctctt
      481 gagaaggaac agaagttaca gaatgacttt gcagaaaagg agagaaagct gcaggagaca
      541 cagatgtcca ccacctcaaa gctggaggaa gctgagcata aggttcagag cctacaaaca
      601 gccctggaaa aaactcgaac agaattattt gacctgaaaa ccaaatacga tgaagaaact
      661 actgcaaagg ccgacgagat tgaaatgatc atgacggacc ttgaaagggc aaaccagagg
      721 gcagaggtgg ctcagagaga ggcggagacc ttaagggaac agctctcatc ggccaatcac
      781 tccctccagc tggcctcaca gatccagaag gcaccagacg tggagcaggc catagaggtg
      841 ctgacccgct ccagcctaga agttgagttg gccgccaagg agcgggagat cgcacagctg
      901 gtggaggacg tgcagagact ccaggccagc ctcaccaagc tgcgggagaa ttcggccagc
      961 cagatctcac agcttgagca gcagctgagc gccaaaaaca gcacactcaa acaactggaa
     1021 gaaaaactca aaggccaggc tgactatgaa gaggtgaaga aagagctgaa cattctgaag
     1081 tccatggagt ttgcaccgtc cgagggcgct gggacacagg atgcggccaa gcccctggag
     1141 gtgctgttgc tggagaagaa ccgctcgctg cagtccgaga acgccgcgct gcgcatctcc
     1201 aacagcgacc tgagcggacg ctgtgcggag ctgcaagtcc gtatcactga ggctgtggcc
     1261 acagccactg agcagagaga gctgatcgcc cgcctggagc aggacctgag catcattcag
     1321 tccatccagc ggcccgatgc cgagggtgcc gctgagcacc gcctggagaa gatcccagag
     1381 cccatcaaag aggccactgc cctattctac ggacctgcag caccagccag cggtgccctc
     1441 ccagagggcc aggtggattc actgctttcc atcatctcca gccagaggga gcgcttccgt
     1501 gcccggaacc aggagcttga ggccgagaac cgcctggccc agcacaccct ccaggccctg
     1561 cagagtgagc tggacagcct gcgcgccgac aacatcaagc tctttgagaa gatcaagttc
     1621 ctgcagagct accctggccg gggcagcggc agtgatgaca cggagctgcg gtactcgtcc
     1681 cagtacgagg agcgcctgga ccccttctcc tccttcagca agcgggagcg gcagaggaag
     1741 tacctgagct tgagtccctg ggacaaggcc accctcagca tggggcgtct ggttctctcc
     1801 aacaagatgg cgcgcaccat cggcttcttc tacacactgt tcctgcactg cctggtcttc
     1861 ctggtgctct acaagctggc atggagcgag agcatggaga gggactgtgc caccttctgc
     1921 gccaagaagt tcgctgacca cctgcacaag ttccacgaga atgacaacgg ggctgcggct
     1981 ggtgacttgt ggcagtagta atgaactagt ccgtaacttg aaagtatttc gatttcttgg
     2041 ctttatatat cttgtggaaa ggacgaagaa agcggtctgc tgtgttcgtc
//
