LOCUS       sequence-280838-        2021 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2021)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2021)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1962
FEATURES             Location/Qualifiers
     source          1..2021
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggccaa tgtgggatcg atgtttcaat attggaagcg ctttgattta
       61 cagcagctgc aggatttgcg caagcaggta gcgccgctgc tgaagagttt ccaaggagag
      121 attgatgcac tgagtaaaag aagcaaggaa gctgaagcag ctttcttgaa tgtctacaaa
      181 agattgattg acgtcccaga tcccgtacca gctttggatc tcggacagca actccagctc
      241 aaagtgcagc gcctgcacga tattgaaaca gagaaccaga aacttaggga aactctggaa
      301 gaatacaaca aggaatttgc tgaagtgaaa aatcaagagg ttacgataaa agcacttaaa
      361 gagaaaatcc gagaatatga acagacactg aagaaccaag ccgaaaccat agctcttgag
      421 aaggaacaga agttacagaa tgactttgca gaaaaggaga gaaagctgca ggagacacag
      481 atgtccacca cctcaaagct ggaggaagct gagcataagg ttcagagcct acaaacagcc
      541 ctggaaaaaa ctcgaacaga attatttgac ctgaaaacca aatacgatga agaaactact
      601 gcaaaggccg acgagattga aatgatcatg acggaccttg aaagggcaaa ccagagggca
      661 gaggtggctc agagagaggc ggagacctta agggaacagc tctcatcggc caatcactcc
      721 ctccagctgg cctcacagat ccagaaggca ccagacgtgg ccatagaggt gctgacccgc
      781 tccagcctag aagttgagtt ggccgccaag gagcgggaga tcgcacagct ggtggaggac
      841 gtgcagagac tccaggccag cctcaccaag ctgcgggaga attcggccag ccagatctca
      901 cagcttgagc agcagctgag cgccaaaaac agcacactca aacaactgga agaaaaactc
      961 aaaggccagg ctgactatga agaggtgaag aaagagctga acattctgaa gtccatggag
     1021 tttgcaccgt ccgagggcgc tgggacacag gatgcggcca agcccctgga ggtgctgttg
     1081 ctggagaaga accgctcgct gcagtccgag aacgccgcgc tgcgcatctc caacagcgac
     1141 ctgagcggac gctgtgcgga gctgcaagtc cgtatcactg aggctgtggc cacagccact
     1201 gagcagagag agctgatcgc ccgcctggag caggacctga gcatcattca gtccatccag
     1261 cggcccgatg ccgagggtgc cgctgagcac cgcctggaga agatcccaga gcccatcaaa
     1321 gaggccactg ccctattcta cggacctgca gcaccagcca gcggtgccct cccagagggc
     1381 caggtggatt cactgctttc catcatctcc agccagaggg agcgcttccg tgcccggaac
     1441 caggagcttg aggccgagaa ccgcctggcc cagcacaccc tccaggccct gcagagtgag
     1501 ctggacagcc tgcgcgccga caacatcaag ctctttgaga agatcaagtt cctgcagagc
     1561 taccctggcc ggggcagcgg cagtgatgac acggagctgc ggtactcgtc ccagtacgag
     1621 gagcgcctgg accccttctc ctccttcagc aagcgggagc ggcagaggaa gtacctgagc
     1681 ttgagtccct gggacaaggc caccctcagc atggggcgtc tggttctctc caacaagatg
     1741 gcgcgcacca tcggcttctt ctacacactg ttcctgcact gcctggtctt cctggtgctc
     1801 tacaagctgg catggagcga gagcatggag agggactgtg ccaccttctg cgccaagaag
     1861 ttcgctgacc acctgcacaa gttccacgag aatgacaacg gggctgcggc tggtgacttg
     1921 tggcagtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1981 tcttgtggaa aggacgaagc acttgaaaca ccggagcagg t
//
