LOCUS       sequence-280847-        1760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1760)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2008
FEATURES             Location/Qualifiers
     source          1..1760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccgggt gccattgaca ttcagcgatg ttgccataga cttctctcag
       61 gaggagtggg aatatttaaa ctcggatcag agggatttgt acagagatgt gatgttggag
      121 aattatacta acttggtctc attggacttt aatttcacaa ctgaaagcaa caagttatct
      181 tcagaaaaaa gaaattatga agtaaatgcg taccatcagg agacatggaa aagaaataaa
      241 accttcaacc ttatgaggtt tattttcaga actgacccac agtacacaat tgaatttggg
      301 agacaacaga gacctaaagt gggatgtttt agtcaaatga tattcaaaaa acataaatcc
      361 cttcctctac ataagagaaa taacacaaga gagaaatcat atgagtgtaa ggaatataag
      421 aagggcttta gaaaatattt gcaccttact gaacatctga gagaccatac tggtgtgata
      481 ccctatgaat gtaatgaatg tggaaaagca tttgtagttt tccagcattt tattagacat
      541 cgaaaaatcc acactgattt gaaaccctat gaatgcaatg gatgtgagaa ggcctttagg
      601 ttttattcac agcttattca gcatcagata attcatactg gtatgaaacc ctatgaatgt
      661 aagcaatgcg ggaaggcttt tagacgtcat tctcacctta cagaacatca gaaaattcat
      721 gttggcttga aaccctttga atgtaaggaa tgtggggaaa cgtttagatt atatcgacat
      781 atgtgtctgc atcagaaaat tcatcatggt gtgaaaccct acaaatgtaa agaatgtgga
      841 aaggcttttg gtcatcgttc aagtctttac caacataaga aaattcattc tggtgagaaa
      901 ccatataaat gtgaacaatg tgaaaaggcc tttgttcgca gctatctact tgttgaacat
      961 caaagaagtc atactggtga gaaacctcat gaatgcatgg aatgtggaaa ggcttttggt
     1021 aagggctcaa gccttcttaa acataagaga attcatagta gtgagaaact ctatgattgt
     1081 aaggattgtg gaaaggcctt ttgtagaggc tctcaactta cacagcatca gagaattcat
     1141 actggtgaga agccacatga atgtaaagaa tgtgggaaga cttttaagct tcattcatat
     1201 cttattcaac atcagataat tcatactgat ttgaagccat atgaatgtaa gcaatgtggg
     1261 aaagccttca gtcgtgttgg agaccttaag acacatcaat caattcatgc tggggagaaa
     1321 ccctatgaat gtaaggaatg tggaaaaacc tttagactta attctcaact aatttatcat
     1381 cagacaattc atactggttt gaaaccctat gtatgtaaag aatgtaagaa ggcctttcgt
     1441 tctatctcag gtctttctca acataagaga attcatactg gtgaaaaacc ctatgaatgt
     1501 aaagaatgtg ataaggcctt taatcgcagt gatcgactta ctcaacatga gacaattcat
     1561 actggtgtga aaccacagaa atgcaaagaa tgtggtaagg cctttagtca ttgctatcaa
     1621 cttagtcaac atcaaagatt tcaccatggt gagagactct taatgtagta atgaactagt
     1681 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgacggt
     1741 atgcaacagg aggcaagtcc
//
