LOCUS       sequence-280850-        3980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2014
FEATURES             Location/Qualifiers
     source          1..3980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggacgcatc atatgatggt actgaggtaa ctgtcgtgat ggaggaaatt
       61 gaggaagcct attgttacac ctctcctggg ccacccaaga agaagaaaaa gtataaaata
      121 catggagaaa agacaaagaa acccaggtct gcttaccttc tgtactatta cgacatctac
      181 ctgaaagtgc agcaggagct cccccacctc cctcagtctg agatcaataa gaagattagt
      241 gagagttgga ggcttctcag cgtggccgag aggagttact acttggagaa agccaaacta
      301 gagaaggaag gtttggatcc taactctaag ctctctgcac tgactgctgt ggttccggac
      361 atcccaggtt tccgcaagat cctcccacgc tcagattata tcatcatccc caagagcagc
      421 ctgcaggagg accggagctg ccctcagcta gagctatgtg tggctcagaa ccagatgtcc
      481 ccgaaaggac ctcctcttgt gtccaacact gccccggaga cagtgcccag ccatgcaggc
      541 atggcagagc agtgcctggc tgtggaggcc ctggctgagg aggtgggagc ccttacccag
      601 tcaggtgctg tacaggagat tgccacctca gagatcctca gccaggatgt gctcctagag
      661 gacgcttccc tagaagtagg ggagagccac caaccttacc agacaagcct ggtaattgaa
      721 gagaccttgg tgaatggctc accagacctc cccactggaa gcctggctgt gccccacccc
      781 caggttgggg agagtgtatc agtggtaaca gtcatgaggg attccagtga gagtagctcc
      841 tctgcaccag ccacacagtt catcatgttg cctctgcctg cctactcggt tgtggagaac
      901 cccacctcca tcaaactgac cactacatat acccgccggg gccatgggac atgcaccagc
      961 ccagggtgct cctttacata tgtcaccagg cacaagccac ctaagtgccc tacctgtggt
     1021 aacttcctag gagggaagtg gatcccaaag gaaaagccag ccaaagtaaa agtggaattg
     1081 gcttctggcg tctcttccaa aggctctgtg gtgaaaagaa atcagcaacc tgtcaccact
     1141 gagcaaaatt cctctaagga aaatgcctcc aaactgactc tggagaattc ggaagctgta
     1201 agccagctcc tgaacgtagc tcctcccaga gaagtaggtg aggagagtga gtgggaggaa
     1261 gtgatcatct ccgatgccca tgttttggtt aaggaagctc ccgggaattg tggtacagca
     1321 gtcactaaga cgccagtcgt caaaagtggt gtgcagcctg aggtcactct ggggacaact
     1381 gacaatgaca gtcctggagc agacgtacca acaccatccg aggggacaag tacctccagt
     1441 ccactccctg ctcctaaaaa acctacagga gctgacctgc ttacccctgg gtccagagct
     1501 ccagagctta aaggcagagc acggggcaag ccctcattac tggctgcagc aagacccatg
     1561 agagcaattt tgccagcccc agttaacgtg gggcgaggca gcagcatggg actgcccagg
     1621 gccaggcagg ccttttccct gagtgataag actccctctg tgaggacttg tggtctgaag
     1681 ccaagcacac tgaagcagct gggccagccc attcaacagc catctggccc tggtgaggtg
     1741 aagctaccaa gtggcccatc caacaggact tctcaggtga aagttgtgga ggtcaagccc
     1801 gatatgtttc ctccatataa gtacagctgc actgtcacat tggatttggg cctggctaca
     1861 tcaagaggcc ggggaaagtg caagaatccc tcttgtagct atgtctacac caacaggcac
     1921 aaacctcgaa tttgtcccag ctgtggtgtt aaccttgcca aagaccggac tgagaaaacc
     1981 accaaggcta tcgaggtgag ctcaccactc ccagatgtac tgaatgccac agagcccctg
     2041 agcacagccc agagggagat ccagcgccag tccacactgc agctgctgcg caaagtcctg
     2101 cagattcctg agaatgagtc agagctggct gaggtcttcg ccttgattca tgaactcaac
     2161 agctctcgac ttatcttgtc caacgtgagt gaggagacag tcaccatcga gcaaacctct
     2221 tggtcgaatt attatgagtc tccgtccacg cagtgccttc tctgtagcag cccattattc
     2281 aaagggggac aaaactccct ggctgggccc caggagtgct ggctgctgac agccagccgt
     2341 ctgcagacag tgactgccca ggtgaagatg tgtctgaacc cccattgtct ggccctgcac
     2401 agcttcatag acatctacac aggtctcttt aatgtgggga acaagctgct ggtaagcctg
     2461 gacttgcttt ttgcaatcag aaatcagatc aagctcggag aggaccccag agtgtccatc
     2521 aatgttgttc tgaagtcggt gcaggagcag acagagaaga ctctgacctc ggaggagctg
     2581 agccagctgc aggagctgct gtgcaatggc tattgggcct ttgagtgcct cactgtccga
     2641 gactacaatg acatgatctg tggcatctgt ggtgtggccc ccaaagtgga aatggctcag
     2701 aggagtgaag agaatgtgct agcactgaag agcgtggagt tcacctggcc tgaattcctg
     2761 ggctctaatg aggtaaatgt ggaggacttt tgggccacga tggagacaga ggtgattgag
     2821 caggtggcat ttcctgccag catccctatc accaaatttg atgcgtctgt tattgccccc
     2881 ttcttcccac cactcatgag aggagctgtg gtcgtcaaca ctgagaaaga caaaaacctg
     2941 gatgtgcagc cagtacctgg cagtggcagt gccttggtga ggctgctcca ggagggcacc
     3001 tgcaagcttg atgagattgg ctcctacagt gaagagaagc tgcagcacct gctaaggcag
     3061 tgtggaatcc cctttggggc agaagactcc aaggaccagc tctgcttctc cttgttggcc
     3121 ctctacgaat ctgtacagaa tggagctaga gctatacggc ccccacgtca cttcacaggt
     3181 ggtaaaatct acaaggtgtg cccccatcag gtggtctgcg gctccaagta tcttgtgcga
     3241 ggtgagagtg cccgtgacca tgtggacctg cttgcctctt cccgccactg gccgcctgtc
     3301 tatgtggtag atatggccac gtcagtggcc ctgtgtgctg acctctgcta cccagagctg
     3361 actaaccaga tgtgggggag gaaccagggc tgtttctcta gccccacaga gccacctgtg
     3421 agtgtgtcct gcccagagct cttggaccag cattatactg tggacatgac agaaactgag
     3481 cactctatcc agcacccagt caccaagact gccacgcggc gcatcgtcca tgcaggccta
     3541 cagcccaatc ctggtgaccc cagtgctggg caccactcct tggccctgtg ccctgaattg
     3601 gcaccttacg caaccatcct ggcctccatc gtggacagca aaccaaacgg tgtccgccag
     3661 cggcccattg ccttcgacaa tgccactcac tattacctct acaaccgcct catggacttc
     3721 ctcaccagcc gcgaaattgt caatcgtcag atccatgaca ttgtacagag ctgccagcct
     3781 ggtgaggtgg tcattcgtga caccctctac cgccttgggg ttgctcagat caagacagag
     3841 acagaggagg agggtgagga agaggaggtg gccgcagtgg cagaatagta atgaactagt
     3901 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaagt
     3961 gtgtgggtgc catggtatac
//
