LOCUS       sequence-280865-        1340 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1340)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2044
FEATURES             Location/Qualifiers
     source          1..1340
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggccct gcagaagcag caggcagctc ggctggccca gggggtgggg
       61 ccattggccc ctgcatgccc gctgctgcca ccgcagcctc ccctgcctga ccaccggacc
      121 ctacaggccc ctgagggggc cttggggaat gttggggctg aggaagagga agatgctgaa
      181 gaagatgagg agaagcggga ggaagccggg gcagaggagg aggcagctga ggagagccgt
      241 ccaggggccc agggccccag ctcgccttct agccagcccc ctggactcca tccccacgag
      301 tggacctacg aggaacaatt caagcagctg tatgagctcg atgcagaccc caagaggaag
      361 gaatttctgg atgacctgtt tagcttcatg caaaagaggg ggacgccagt gaaccgcgtg
      421 cccatcatgg cgaagcaggt gctcgacctg tacgctctgt ttcgcctggt gaccgccaag
      481 ggcggcctgg tggaagtcat caaccgcaaa gtgtggcggg aagtcacgcg cggcctcagc
      541 ctacccacca ccatcacctc ggccgccttc actctacgca cccagtacat gaagtacctg
      601 tacccgtacg agtgcgagac tcgagcgctc agctccccag gggagctcca ggccgccata
      661 gacagcaatc ggcgcgaggg ccgtcgccag gcttacaccg ctactccgct cttcggcttg
      721 gcagggccgc cccctcgggg cgctcaggac ccagccttgg gtcccggccc cgcccctccg
      781 gcgacccagt ccagccctgg cccagcccag ggttccacct ccggcctgcc agcgcatgca
      841 tgcgctcagc tgagtccaag ccctattaag aaagaggaga gtggaattcc aaacccttgt
      901 ctggcactgc ctgtgggcct ggcactggga cctacacggg agaaattggc accagaggag
      961 cccccagaga agagagctgt gctgatgggg cctatggacc cacctcgacc ttgcatgccc
     1021 cccagtttcc tgccccgtgg caaggttccc ctgagggaag agcggctgga tgggcctctt
     1081 aatctggcag gcagtggcat cagcagtatc aacatggccc tagagatcaa cggggtggtc
     1141 tacactggtg tcctctttgc ccgccgccag cctgtgccag cttcccaggg tccaaccaac
     1201 cctgcacccc caccctccac agggccccct tccagcatct tgccctagta atgaactagt
     1261 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgatgtt
     1321 gcagggcgtt tctgaagatg
//
