LOCUS       sequence-280868-        2213 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2213)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2213)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2050
FEATURES             Location/Qualifiers
     source          1..2213
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggagct gatgctgctc agcgagattg ctgacccgac gcgtttcttc
       61 accgacaacc tgcttagccc ggaggactgg ggtctgcaga acagcacctt gtattctggc
      121 ctagatgaag tggccgagga gcagacgcag ctcttccgtt gcccggagca ggatgtcccg
      181 tttgacggca gctccctgga cgtggggatg gatgtcagcc cctctgagcc cccatgggaa
      241 ctcctgccga tcttcccaga tcttcaggtg aagtctgagc catcttcccc ctgctcttcc
      301 tcctccctca gctccgagtc atcgcgtctc tccacagagc catccagcga ggctcttggg
      361 gtaggggagg tgctccatgt gaagacagag tccttggcac ccccactgtg tctcctggga
      421 gatgacccaa catcctcatt tgaaaccgtc cagatcaacg ttatccccac ctctgatgat
      481 tcctcagatg tccagaccaa gatagaacct gtctctccat gttcttccgt caactctgag
      541 gcctccctgc tctcagccga ctcctccagc caggctttta taggagagga ggtcctggaa
      601 gtgaagacag agtccctgtc cccttcagga tgcctcctgt gggatgtccc agccccctca
      661 cttggagctg tccagatcag catgggccca tcccttgatg gctcctcagg caaagccctg
      721 cccacccgga agccgccact gcagcccaaa cctgtagtgc taaccactgt cccaatgcca
      781 tccagagctg tgcctcccag caccacagtc cttctgcagt ccctcgtcca gccaccccca
      841 gtgtccccag ttgtcctcat ccagggtgct attcgagtcc agcctgaagg gccggctccc
      901 tctctaccac ggcctgagag gaagagcatc gttcccgctc ctatgcctgg aaactcctgc
      961 ccgcctgaag tggatgcaaa gctgctgaag cggcagcagc gaatgatcaa gaaccgggag
     1021 tcagcctgcc agtcccggag aaagaagaaa gagtatctgc agggactgga ggctcggctg
     1081 caagcagtac tggctgacaa ccagcagctc cgccgagaga atgctgccct ccggcggcgg
     1141 ctggaggccc tgctggctga aaacagcgag ctcaagttag ggtctggaaa caggaaggtg
     1201 gtctgcatca tggtcttcct tctcttcatt gccttcaact ttggacctgt cagcatcagt
     1261 gagcctcctt cagctcccat ctctcctcgg atgaacaagg gggagcctca accccggaga
     1321 cacttgctgg ggttctcaga gcaagagcca gttcagggag ttgaacctct ccaggggtcc
     1381 tcccagggcc ctaaggagcc ccagcccagc cccacagacc agcccagttt cagcaacctg
     1441 acagccttcc ctgggggcgc caaggagcta ctactaagag acctagacca gctcttcctc
     1501 tcctctgatt gccggcactt caaccgcact gagtccctga ggcttgctga cgagttgagt
     1561 ggctgggtcc agcgccacca gagaggccgg aggaagatcc ctcagagggc ccaggagaga
     1621 cagaagtctc agccacggaa gaagtcacct ccagttaagg cagtccccat ccaaccccct
     1681 ggacccccag aaagggattc tgtgggccag ctgcaactat atcgccaccc agaccgttcg
     1741 cagccagcat tcttggatgc aattgaccga cgggaagaca cattttatgt tgtctctttc
     1801 cgaagggacc acctgctgct cccagccatc agccacaaca agacctcccg gcccaagatg
     1861 tccctggtga tgcctgccat ggcccccaat gagaccctgt caggccgtgg ggccccgggg
     1921 gactatgagg agatgatgca gatcgagtgt gaggtcatgg acaccagggt gattcacatc
     1981 aagacctcca cagtgccccc ctcgctccga aaacagccat ccccaacccc aggcaatgcc
     2041 acaggtggcc ccttgccagt ctctgcagcc agccaggccc accaggcctc ccaccagccc
     2101 ctctacctca atcatcccta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     2161 tggctttata tatcttgtgg aaaggacgag ttcaattccc tagtgcggca ctt
//
