LOCUS       sequence-280874-        2222 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2053
FEATURES             Location/Qualifiers
     source          1..2222
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcctcgcc ggctgacagc tgtatccagt tcacccgcca tgccagtgat
       61 gttcttctca accttaatcg tctccggagt cgagacatct tgactgatgt tgtcattgtt
      121 gtgagccgtg agcagtttag agcccataaa acggtcctca tggcctgcag tggcctgttc
      181 tatagcatct ttacagacca gttgaaatgc aaccttagtg tgatcaatct agatcctgag
      241 atcaaccctg agggattctg catcctcctg gacttcatgt acacatctcg gctcaatttg
      301 cgggagggca acatcatggc tgtgatggcc acggctatgt acctgcagat ggagcatgtt
      361 gtggacactt gccggaagtt tattaaggcc agtgaagcag agatggtttc tgccatcaag
      421 cctcctcgtg aagagttcct caacagccgg atgctgatgc cccaagacat catggcctat
      481 cggggtcgtg aggtggtgga gaacaacctg ccactgagga gcgcccctgg gtgtgagagc
      541 agagcctttg cccccagcct gtacagtggc ctgtccacac cgccagcctc ttattccatg
      601 tacagccacc tccctgtcag cagcctcctc ttctccgatg aggagtttcg ggatgtccgg
      661 atgcctgtgg ccaacccctt ccccaaggag cgggcactcc catgtgatag tgccaggcca
      721 gtccctggtg agtacagccg gccgactttg gaggtgtccc ccaatgtgtg ccacagcaat
      781 atctattcac ccaaggaaac aatcccagaa gaggcacgaa gtgatatgca ctacagtgtg
      841 gctgagggcc tcaaacctgc tgccccctca gcccgaaatg ccccctactt cccttgtgac
      901 aaggccagca aagaagaaga gagaccctcc tcggaagatg agattgccct gcatttcgag
      961 ccccccaatg cacccctgaa ccggaagggt ctggttagtc cacagagccc ccagaaatct
     1021 gactgccagc ccaactcgcc cacagagtcc tgcagcagta agaatgcctg catcctccag
     1081 gcttctggct cccctccagc caagagcccc actgacccca aagcctgcaa ctggaagaaa
     1141 tacaagttca tcgtgctcaa cagcctcaac cagaatgcca aaccagaggg gcctgagcag
     1201 gctgagctgg gccgcctttc cccacgagcc tacacggccc cacctgcctg ccagccaccc
     1261 atggagcctg agaaccttga cctccagtcc ccaaccaagc tgagtgccag cggggaggac
     1321 tccaccatcc cacaagccag ccggctcaat aacatcgtta acaggtccat gacgggctct
     1381 ccccgcagca gcagcgagag ccactcacca ctctacatgc accccccgaa gtgcacgtcc
     1441 tgcggctctc agtccccaca gcatgcagag atgtgcctcc acaccgctgg ccccacgttc
     1501 cctgaggaga tgggagagac ccagtctgag tactcagatt ctagctgtga gaacggggcc
     1561 ttcttctgca atgagtgtga ctgccgcttc tctgaggagg cctcactcaa gaggcacacg
     1621 ctgcagaccc acagtgacaa accctacaag tgtgaccgct gccaggcctc cttccgctac
     1681 aagggcaacc tcgccagcca caagaccgtc cataccggtg agaaacccta tcgttgcaac
     1741 atctgtgggg cccagttcaa ccggccagcc aacctgaaaa cccacactcg aattcactct
     1801 ggagagaagc cctacaaatg cgaaacctgc ggagccagat ttgtacaggt ggcccacctc
     1861 cgtgcccatg tgcttatcca cactggtgag aagccctatc cctgtgaaat ctgtggcacc
     1921 cgtttccggc accttcagac tctgaagagc cacctgcgaa tccacacagg agagaaacct
     1981 taccattgtg agaagtgtaa cctgcatttc cgtcacaaaa gccagctgcg acttcacttg
     2041 cgccagaagc atggcgccat caccaacacc aaggtgcaat accgcgtgtc agccactgac
     2101 ctgcctccgg agctccccaa agcctgctag taatgaacta gtccgtaact tgaaagtatt
     2161 tcgatttctt ggctttatat atcttgtgga aaggacgatc gcatccgcga cagacgcatg
     2221 tg
//
