LOCUS       sequence-280898-        1571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1571)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2151
FEATURES             Location/Qualifiers
     source          1..1571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             148..177
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             151..180
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 accgccacca tgctggacat gggagatagg aaagaggtga aaatgatccc caagtcctcg
       61 ttcagcatca acagcctggt gcccgaggcg gtccagaacg acaaccacca cgcgagccac
      121 ggccaccaca acagccacca cccccagcac caccaccacc accaccacca tcaccaccac
      181 ccgccgccgc ccgccccgca accgccgccg ccgccgcagc agcagcagcc gccgccgccg
      241 ccgcccccgg caccgcagcc cccccagacg cggggcgccc cggccgccga cgacgacaag
      301 ggcccccagc agctgctgct cccgccgccg ccaccgccac caccggccgc cgccctggac
      361 ggggctaaag cggacgggct gggcggcaag ggcgagccgg gcggcgggcc gggggagctg
      421 gcgcccgtcg ggccggacga gaaggagaag ggcgccggcg ccggggggga ggagaagaag
      481 ggggcgggcg agggcggcaa ggacggggag gggggcaagg agggcgagaa gaagaacggc
      541 aagtacgaga agccgccgtt cagctacaac gcgctcatca tgatggccat ccggcagagc
      601 cccgagaagc ggctcacgct caacggcatc tacgagttca tcatgaagaa cttcccttac
      661 taccgcgaga acaagcaggg ctggcagaac tccatccgcc acaatctgtc cctcaacaag
      721 tgcttcgtga aggtgccgcg ccactacgac gacccgggca agggcaacta ctggatgctg
      781 gacccgtcga gcgacgacgt gttcatcggc ggcaccacgg gcaagctgcg gcgccgctcc
      841 accacctcgc gggccaagct ggccttcaag cgcggtgcgc gcctcacctc caccggcctc
      901 accttcatgg accgcgccgg ctccctctac tggcccatgt cgcccttcct gtccctgcac
      961 cacccccgcg ccagcagcac tttgagttac aacggcacca cgtcggccta ccccagccac
     1021 cccatgccct acagctccgt gttgactcag aactcgctgg gcaacaacca ctccttctcc
     1081 accgccaacg gcctgagcgt ggaccggctg gtcaacgggg agatcccgta cgccacgcac
     1141 cacctcacgg ccgccgcgct agccgcctcg gtgccctgcg gcctgtcggt gccctgctct
     1201 gggacctact ccctcaaccc ctgctccgtc aacctgctcg cgggccagac cagttacttt
     1261 ttcccccacg tcccgcaccc gtcaatgact tcgcagagca gcacgtccat gagcgccagg
     1321 gccgcgtcct cctccacgtc gccgcaggcc ccctcgaccc tgccctgtga gtctttaaga
     1381 ccctctttgc caagttttac gacgggactg tctgggggac tgtctgatta tttcacacat
     1441 caaaatcagg ggtcttcttc caacccttta atacattagt aatgaactag tccgtaactt
     1501 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaggt aaatacggat
     1561 ctagctgagc g
//
