LOCUS       sequence-280907-        1274 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1274)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1274)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2186
FEATURES             Location/Qualifiers
     source          1..1274
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaggagaa accctcaggg cccatcccag acatgctggc cactgcagag
       61 cccagctcca gtgagaccga caaggaggtg ttgtccccgg ctgtgccagc tgcagcccct
      121 tcctcctcca tgtcggagga gccaggccct gagcaggcag ccacaccgcc agtgtgggaa
      181 cgtggagggg ctggagggat gcagcagggc tcctccccag ccccagacag ctgccagcct
      241 ggccccggac ccagccctgg cctgaccagc atagtctccg ggaccagcga ggacctgcgg
      301 cctcccagac gacgcccacc tccagggaag caaatccctt gctccagccc tggctgctgc
      361 ctcagttttc ccagcgtccg tgacctggca cagcatctgc gaacccactg cccgcccaca
      421 cagtccctgg aaggcaagct cttccgctgc tcagccctga gctgcaccga gaccttcccc
      481 agcatgcagg agctggtggc tcacagcaaa ctgcactaca aacccaatcg ctacttcaag
      541 tgtgagaact gcctcctgcg cttccgcacg caccgctcgc tcttcaagca cctgcatgtt
      601 tgcgcggagc atgcgcagag cccagccccg ccgccacccc cggccctgga ccgagagccg
      661 cccgcgccgg agcgcccccc ggaggttgac cccgcatcag cgccgggcct gccgttcccg
      721 ctgctggaac ccttcacgac ccccgcccct gcccccaccg gaccgttcct gccctacttg
      781 aaccctgcgc cctttggcct aagccccccg cgcctgcgcc ccttcctggc cgctgcaccc
      841 gggccgccgg cttccagcgc cgccgtctgg aaaaagagcc aaggtgctgg cagcagcccc
      901 cgaagacccc agggcggctc cgacgcgccc tcagggcacg cggccccgag ccgcatcgtg
      961 tgggagcaca cacgcggccg ctactcgtgc atgcagtgcg ccttctccac ggcctcgcgg
     1021 cccgccatga ccctgcacct ggaggaccac cgccccggcg cccccgcggc ccccgcggcc
     1081 gggccgccgc gccccgacgc ccccgcggat ccggccccgc ttgcacccaa ggtgtcgccg
     1141 ctgctgtcag agggggagct tccagtgttc tcgcagctct agtaatgaac tagtccgtaa
     1201 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga tacatcgagt
     1261 cttggaggaa acta
//
