LOCUS       sequence-280919-        1427 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2258
FEATURES             Location/Qualifiers
     source          1..1427
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgcagca ggcggagagc ttggaagcgg agagcaacct gccccgggag
       61 gcgctggaca cggaggaggg cgaattcatg gcttgcagcc cggtggccct ggacgagagc
      121 gacccagact ggtgcaagac ggcgtcgggc cacatcaagc ggccgatgaa cgcgttcatg
      181 gtatggtcca agatcgaacg caggaagatc atggagcagt ctccggacat gcacaacgcc
      241 gagatctcca agaggctggg caagcgctgg aaaatgctga aggacagcga gaagatcccg
      301 ttcatccggg aggcggagcg gctgcggctc aagcacatgg ccgactaccc cgactacaag
      361 taccggcccc ggaaaaagcc caaaatggac ccctcggcca agcccagcgc cagccagagc
      421 ccagagaaga gcgcggccgg cggcggcggc gggagcgcgg gcggaggcgc gggcggtgcc
      481 aagacctcca agggctccag caagaaatgc ggcaagctca aggcccccgc ggccgcgggc
      541 gccaaggcgg gcgcgggcaa ggcggcccag tccggggact acgggggcgc gggcgacgac
      601 tacgtgctgg gcagcctgcg cgtgagcggc tcgggcggcg gcggcgcggg caagacggtc
      661 aagtgcgtgt ttctggatga ggacgacgac gacgacgacg acgacgacga gctgcagctg
      721 cagatcaaac aggagccgga cgaggaggac gaggaaccac cgcaccagca gctcctgcag
      781 ccgccggggc agcagccgtc gcagctgctg agacgctaca acgtcgccaa agtgcccgcc
      841 agccctacgc tgagcagctc ggcggagtcc cccgagggag cgagcctcta cgacgaggtg
      901 cgggccggcg cgacctcggg cgccgggggc ggcagccgcc tctactacag cttcaagaac
      961 atcaccaagc agcacccgcc gccgctcgcg cagcccgcgc tgtcgcccgc gtcctcgcgc
     1021 tcggtgtcca cctcctcgtc cagcagcagc ggcagcagca gcggcagcag cggcgaggac
     1081 gccgacgacc tgatgttcga cctgagcttg aatttctctc aaagcgcgca cagcgccagc
     1141 gagcagcagc tggggggcgg cgcggcggcc gggaacctgt ccctgtcgct ggtggataag
     1201 gatttggatt cgttcagcga gggcagcctg ggctcccact tcgagttccc cgactactgc
     1261 acgccggagc tgagcgagat gatcgcgggg gactggctgg aggcgaactt ctccgacctg
     1321 gtgttcacat attagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1381 tatatatctt gtggaaagga cgatcggacg gcgtggtagt atctgac
//
