LOCUS       sequence-280922-        1697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1697)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2268
FEATURES             Location/Qualifiers
     source          1..1697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggggtttct ccatgttggt caggatggtc tcgaactccc gacatcaggt
       61 gatccgcctg cctcggcctc ccaaagtgct gggattacag gtgtgagcca ccgcactcag
      121 cctccctgtt ttgaaggatt gacttctaaa gacttggtac gtgaggaaaa aacacggaag
      181 aggaagagga aagcaaagga gtcagggatg gctcttcttc agggtctact gacattcagg
      241 gatgtggcca tagaattctc tcaggaggag tggaaatgcc tggaccctgc tcagaggact
      301 ctatacagag acgtgatgct ggagaattat aggaacctgg tctccctgga tacctcttcc
      361 aaatgcatga tgaagatgtt ctcatcaaca ggacaaggca atacagaagt ggtccacaca
      421 gggacattgc aaatacatgc aagtcatcac attggagata cttgcttcca ggaaattgag
      481 aaagatattc atgactttgt gtttcagtgg caagaaaatg aaacaaatgg ccatgaagca
      541 ctcatgacaa aaaccaaaaa gttgatgagt agtacagagc gacatgatca aaggcatgct
      601 ggaaacaaac ctattaaaaa tgagcttgga tcaagctttc attcgcatct gcctgaagtg
      661 cacatatttc accccgaagg gaaaattggt aatcaagttg agaaggctat caacgatgct
      721 ttctcagttt cagcatccca acgaatttcc tgtaggccaa aaactcgtat ttctaataag
      781 tataggaata atttcctcca gtcttcatta ctcacacaaa aacgggaagt acacacaaga
      841 gaaaaatctt tccaacgtaa tgagagtggc aaagccttta atggtagctc actcttaaaa
      901 aaacatcaga taatccattt aggagacaaa cagtataaat gtgatgtatg cggcaaggac
      961 tttcatcaga agcgatacct tgcatgccat agatgtcaca ctggtgagaa tccttacacg
     1021 tgtaatgagt gtggcaagac attcagtcac aattcagccc tgttagttca caaggcaatt
     1081 catactggag agaaacctta caagtgtaat gaatgtggca aggtttttaa tcaacaatca
     1141 aaccttgcac gtcatcatag agttcatact ggagagaaac cttacaaatg tgaagaatgt
     1201 gacaaagttt tcagtcgcaa atcacacctt gaaagacata ggagaattca cactggagag
     1261 aaaccataca aatgtaaggt ttgtgacaag gctttcagac gtgattcaca cctggcacaa
     1321 catactgtaa ttcacactgg agagaaacct tacaagtgta atgagtgtgg caagaccttt
     1381 gttcaaaatt catctcttgt aatgcataag gtcattcata ctggagagaa acgttacaag
     1441 tgtaatgaat gtggcaaggt ttttaatcac aaatcaaacc ttgcatgtca tcgtagactt
     1501 catactggag agaaacctta caagtgtaat gaatgtggca aggtttttaa tcgaaaatca
     1561 aaccttgaac gtcatcatag acttcatact ggaaagaaat cttagtaatg aactagtccg
     1621 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaagccgat
     1681 tgatcaacgt agatgta
//
