LOCUS       sequence-280925-        4100 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 4100)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 4100)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2281
FEATURES             Location/Qualifiers
     source          1..4100
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagagccg ggatcccgcc cagccgatgt cgcctggaga agcgacgcag
       61 agtggcgccc ggcctgccga ccgctatggc ctattgaagc acagtcgcga gttcttggac
      121 ttcttctggg acattgcgaa gcctgagcag gagacgcgac ttgcggccac ggagaagctg
      181 ctggagtatc tgcgtggcag gccgaagggg tccgagatga aatatgccct gaagcgtcta
      241 atcacgggac tcggggtcgg gcgagaaaca gcccggccct gctacagttt ggccctggca
      301 cagctgttac agtcttttga agacctcccc ttgtgcagca tcctgcagca gatacaagaa
      361 aaatatgacc tgcatcaggt gaagaaggca atgctgagac ctgctctctt tgcaaacctg
      421 tttggagtgc tcgccctctt tcagtcaggt cggctggtga aggaccagga ggcactgatg
      481 aagtcggtga agctgctgca ggccctggcc cagtaccaaa accacttgca ggagcagccc
      541 cggaaggccc tggtggacat cctctccgag gtctcgaagg ccacattgca ggagatcctg
      601 ccggaggtcc tcaaagccga cttgaatata atactcagct cccctgaaca gctagagctc
      661 ttcctcctgg cccagcagaa ggtgccctcc aagctcaaga agctggtggg atccgtgaac
      721 ctattctcag atgagaatgt ccccaggctg gtgaatgtgc tgaagatggc cgcctcctct
      781 gtgaagaagg accgcaagct gcccgccatt gctctggacc tgctccgcct ggcactcaag
      841 gaagacaagt tcccacggtt ctggaaggag gtggtggaac aagggctgct gaagatgcag
      901 ttctggccag ccagctacct gtgtttccgc ctgctgggcg cggccctgcc cctgctgacc
      961 aaggagcagc tgcacctggt gatgcaggga gacgtgatcc gccattacgg ggagcacgtg
     1021 tgcactgcta agctcccaaa gcagttcaag tttgccccag agatggacga ttacgtgggc
     1081 accttcctag aggggtgcca ggatgaccct gagcggcagc tggccgtgct agtggccttc
     1141 tcatctgtca ccaaccaagg cctccctgtc acgcctactt tctggcgggt cgtgcggttc
     1201 ctgagccctc cggccctgca gggctatgtg gcctggctgc gggccatgtt tctccagcca
     1261 gacctggact ccttggttga cttcagcacc aacaaccaga agaaagccca ggattcatcg
     1321 ctccacatgc ctgagcgagc tgtgttccgg ctgaggaaat ggatcatctt tcgattggtg
     1381 agcattgtgg acagcctgca cctggagatg gaggaggcct tgactgagca ggtggccagg
     1441 ttttgtttgt tccactcgtt ctttgtcaca aagaagccca catcccagat ccctgagaca
     1501 aagcacccgt tctccttccc tttggaaaac caggcccgag aggctgtcag cagtgccttc
     1561 ttcagtctgt tgcagaccct cagcacgcag ttcaagcagg caccgggcca gacccagggt
     1621 gggcagccct ggacctacca cctggtgcag ttcgcagacc tcctgttgaa tcacagccac
     1681 aacgtgacca ccgtgacacc cttcactgcg cagcagcgcc aggcctggga ccggatgctg
     1741 cagactctga aggagctgga ggcccactcc gcagaggcca gggctgctgc cttccagcac
     1801 cttctgctcc tcgtgggcat ccacctcctc aagtcccctg cagagagctg tgacctgctg
     1861 ggtgacatcc agacctgcat caggaaaagt ctgggagaga agccccgccg gagccgcacc
     1921 aagaccatcg acccccagga acccccgtgg gtagaggtgc tggtggagat cttgctggcc
     1981 ctgttggccc agcccagcca cctcatgcgc caggtggccc ggagcgtgtt tggccacatc
     2041 tgctcccacc tgaccccgcg tgccctgcag ctaattctgg atgtgctgaa ccccgagacc
     2101 agtgaggatg agaatgaccg tgtggtggtg acggacgatt ctgatgagcg gcggctgaag
     2161 ggtgcagagg acaagagcga ggaaggtgag gacaacagaa gctcagagag tgaagaggag
     2221 agcgaggggg aggagagcga ggaggaggag cgcgacgggg acgtggatca gggcttccgg
     2281 gaacagctga tgaccgtgct gcaggctggg aaggcgctgg gtggagagga cagtgagaac
     2341 gaggaggagc tgggggatga ggccatgatg gccctggacc agagcctcgc cagcctcttt
     2401 gccgagcaga agctgcgtat ccaggcccgg cgagacgaga agaacaagct gcagaaggag
     2461 aaggctctgc ggcgcgactt ccagatccgg gtgctggacc tggtggaggt gctagtgacc
     2521 aagcagcccg agaatgccct ggtcctggag ctgctggagc cgctgctgag catcatccgg
     2581 cgcagcctgc gcagcagcag ctccaaacag gagcaggacc ttctgcacaa gacggcgcgc
     2641 atcttcacgc accacctgtg ccgtgcccgg cgctactgcc acgacttggg tgagcgcgca
     2701 ggggccctgc acgcccaggt ggagcggttg gtgcagcagg ctggccgcca gcccgactcc
     2761 cccaccgccc tctaccactt caacgcctct ctctacctgc tccgggtctt gaagggcaac
     2821 actgctgagg gctgcgtgca tgagacacag gagaagcaga aagctggcac tgaccccagc
     2881 cacatgccca cgggcccgca ggctgccagc tgcttggact tgaacctggt gacccgggtg
     2941 tactcgacag cactgagctc cttcctgacc aagcgcaaca gccccctcac agttcccatg
     3001 ttcctcagcc tcttctcccg gcacccggtg ctctgtcaga gcctgctccc catcctggtc
     3061 cagcatatca cgggcccggt gcggccccgt catcaggcct gcctgctgct ccagaagacc
     3121 ctgtccatgc gggaggtgag gtcgtgcttt gaggaccccg agtggaagca gctgatgggc
     3181 caggtcctag caaaggtcac cgagaacttg cgcgtgctgg gggaggcgca gaccaaggcg
     3241 cagcatcagc aggcactgtc ctccctggag ctgctcaacg ttctcttcag gacctgcaaa
     3301 catgagaagc tgaccttgga cctgacggtg ctcctgggtg tgctgcaggg gcaacagcag
     3361 agcctacagc agggggcaca ctccaccggc tccagccgcc tgcacgacct ctactggcag
     3421 gccatgaaaa ccctgggagt ccagcgcccc aagttggaga agaaggatgc caaggagatc
     3481 cccagtgcca cccagagccc catcagtaag aagcggaaga aaaagggatt cttgccagag
     3541 acgaagaagc gcaagaaacg caagtcagag gatggcacgc cagcggagga tggcacacct
     3601 gcagccaccg gcgggagcca gccccccagc atgggcagga agaagaggaa caggacaaag
     3661 gctaaggtcc cagcccaggc aaacgggacg ccaaccacca agagtccagc ccctggcgcc
     3721 cccacccgga gccccagcac ccctgccaaa tccccaaaac tgcagaagaa aaaccagaag
     3781 ccgtcccagg tgaatggagc tcccgggtcc cccacggaac ctgcaggcca aaagcagcat
     3841 cagaaggctc ttcccaaaaa gggggtcttg ggcaaatcac cactgtccgc gctggcacgg
     3901 aaaaaggcaa ggctgtcttt ggtcatcagg agtcccagcc tgcttcagag tggggccaag
     3961 aagaaagcac agactctgag attcacaatc agcagctcta aaaaatagta atgaactagt
     4021 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaagac
     4081 tcgtctagag ttaagagcgg
//
