LOCUS       sequence-28094-e         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PhyB-mCherry with prenylation sequence from Kras, for 
            red-light-induced recruitment of PIF to the plasma membrane.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL149
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(338..356)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(339..356)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(376..393)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    382..606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(739..758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
       61 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      121 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      181 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      241 ctgtacaagg ctagcggtaa aaagaagaaa aagaagtcaa agacaaagtg tgtaattatg
      301 taagcggccg ctcgagcatg catctagagg gccctattct atagtgtcac ctaaatgcta
      361 gagctcgctg atcagcctcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
      421 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
      481 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
      541 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct
      601 ctatggcttc tgaggcggaa agaaccagct ggggctctag ggggtatccc cacgcgccct
      661 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
      721 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
      781 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
      841 ggcacctcga ccccaaaaaa cttgatta
//
