LOCUS       sequence-280949-        2069 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2069)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2069)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2374
FEATURES             Location/Qualifiers
     source          1..2069
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgagga ctggctggac tgcccggccc tgggccctgg ctggaagcgc
       61 cgcgaagtct ttcgcaagtc aggggccacc tgtggacgct cagacaccta ttaccagagc
      121 cccacaggag acaggatccg aagcaaagtt gagctgactc gatacctggg ccctgcgtgt
      181 gatctcaccc tcttcgactt caaacaaggc atcttgtgct atccagcccc caaggcccat
      241 cccgtggcgg ttgccagcaa gaagcgaaag aagccttcaa ggccagccaa gactcggaaa
      301 cgtcaggttg gaccccagag tggtgaggtc aggaaggagg ccccgaggga tgagaccaag
      361 gctgacactg acacagcccc agcttcattc cctgctcctg ggtgctgtga gaactgtgga
      421 atcagcttct caggggatgg cacccaaagg cagcggctca aaacgttgtg caaagactgt
      481 cgagcacaga gaattgcctt caaccgggaa cagagaatgt ttaagcgtgt gggctgtggg
      541 gagtgtgcag cctgccaggt aacagaagac tgtggggcct gctccacctg cctcctgcag
      601 ctgccccatg atgtggcatc ggggctgttc tgcaagtgtg aacggagacg ctgcctccgg
      661 attgtggaaa ggagccgagg gtgtggagta tgccggggct gtcagaccca agaggattgt
      721 ggccattgcc ccatctgcct tcgccctccc cgccctggtc tcaggcgcca gtggaaatgt
      781 gtccagcgac gttgcctacg gggtaaacat gcccgccgca agggaggctg tgactccaag
      841 atggctgcca ggcggcgccc cggagcccag ccactgcctc caccaccccc atcacagtcc
      901 ccagagccca cagagccgca ccccagagcc ctggccccct cgccacctgc cgagttcatc
      961 tattactgtg tagacgagga cgagctacag ccctacacga accgccggca gaaccgcaag
     1021 tgcggggcct gtgcagcctg cctacggcgg atggactgtg gccgctgcga cttctgctgc
     1081 gacaagccca aattcggggg cagcaaccag aagcgccaga agtgtcgttg gcgccaatgc
     1141 ctgcagtttg ccatgaagcg gctgctgccc agtgtctggt cagagtctga ggatggggca
     1201 ggatcgcccc caccttaccg tcgtcgaaag aggcccagct ctgcccgacg gcaccatctt
     1261 ggccctacct tgaagcccac cttggctaca cgcacagccc aaccagacca tacccaggct
     1321 ccaacgaagc aggaagcagg tggtggcttt gtgctgcccc cgcctggcac tgaccttgtg
     1381 tttttacggg aaggcgcaag cagtcctgtg caggtgccgg gccctgttgc agcttccaca
     1441 gaagccctgt tgcaggaggc ccagtgctct ggcctgagtt gggttgtggc cttaccccag
     1501 gtgaagcaag agaaggcgga tacccaggac gagtggacac caggcacagc tgtcctgact
     1561 tctcccgtat tggtgcctgg ctgccctagc aaggcagtag acccaggcct gccttctgtg
     1621 aagcaagagc cacctgaccc agaggaggac aaggaggaga acaaggatga ttctgcctcc
     1681 aaattggccc cagaggaaga ggcaggaggg gctggcacac ccgtgatcac ggagattttc
     1741 agcctgggtg gaacccgctt ccgagataca gcagtctggt tgccaagtct gcagggcagg
     1801 cactcgggaa gggaagatgg atgtaaagtg tgggagaccg aggacacagt ggagcccacg
     1861 agcacgagct ggaacccacg aggatggcct ggaacccatg tcagtctctc accacctcca
     1921 gcttcgatga tgtgggtgtc ctgcagaaga agctggtgcc cttcctcaca gagttagtaa
     1981 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     2041 gacgattgca tctttcggga atcaagtaa
//
