LOCUS       sequence-280958-        1652 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1652)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1652)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2422
FEATURES             Location/Qualifiers
     source          1..1652
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaccccgc aggggcagca gacccctcag tgcctcccaa tcctttgact
       61 cacctgagcc tgcaggacag atcagagatg cagctgcaga gcgaagccga caggcggagc
      121 ctcccgggca cttggaccag gtcatcccca gagcacacca ccattctgag gggaggcgtg
      181 cgcaggtgcc tgcagcaaca gtgtgaacag actgtgcgga tcctgcatgc caaggtggcc
      241 cagaaatcat acggaaatga gaagcggttc ttctgccccc cgccctgtgt ctacctctcg
      301 gggcctggct ggagggtgaa gccagggcag gatcaagctc accaggcggg ggaaacgggg
      361 cccacggtct gcggttacat gggactggac agcgcgtccg gcagcgccac tgagacgcag
      421 aagctgaatt tcgagcagca gccggactcc agggaattcg gctgcgccaa gaccctgtac
      481 atctcagatg cagacaagag gaagcacttt cggctggtgc tgcggctggt gctgcgcggg
      541 ggccgggagc tgggtacctt ccacagccgc cttatcaagg tcatctcgaa gccctcgcag
      601 aagaagcagt cgctgaaaaa caccgatctg tgcatatcct ccggctcaaa ggtctccctc
      661 ttcaaccgcc tgcgctctca gacggtctcc acacgctacc tctctgtgga ggatggggcc
      721 tttgtggcca gtgcacgaca gtgggctgcc ttcacgctcc acctggctga tgggcactct
      781 gcccaaggag acttcccacc gcgagagggc tacgttcgct atggctccct ggtgcagctc
      841 gtctgcacgg tcaccggcat cacactacct cccatgatca tccgtaaagt agcaaaacag
      901 tgtgcgctcc ttgatgtgga tgagcccatc tcccagctgc acaagtgtgc attccagttt
      961 ccaggcagtc ccccaggagg gggtggcacc tacttatgcc ttgccacaga gaaggtggtg
     1021 caatttcagg cctctccctg ccccaaggag gcgaacaggg ctctgcttaa cgacagctct
     1081 tgctggacca tcatcggcac cgagtcggtg gaattttcct tcagcaccag cctggcgtgt
     1141 accctggagc cggtcactcc ggtgcctctc atcagcaccc tagagctgag cggcgggggc
     1201 gacgtggcca cgctggagct ccacggagag aacttccacg cggggctcaa ggtgtggttt
     1261 ggggacgtgg aggcagaaac catgtacagc ccgcggtccc tggtgtgcgt ggtgccggac
     1321 gtggcggcct tctgcagcga ctggcgctgg ctgcgcgctc ccatcacaat ccccatgagc
     1381 ctggtgcgcg ccgacgggct cttctaccct agtgccttct ccttcaccta caccccggaa
     1441 tacagcgtgc ggccgggtca ccccggcgtc cccgagcccg ccaccgacgc cgacgcgctc
     1501 ctggagagca tccatcagga gttcacgcgc accaacttcc acctcttcat ccagacttag
     1561 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1621 aaggacgata atggaaatta gataacccta ag
//
