LOCUS       sequence-28096-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Tiam DH-PH domains fused to mYFP-PIF6, for optogenetic 
            recruitment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL188
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(370..388)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(371..388)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(408..425)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    414..638
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctctcggcat ggacgagctg tacaagggat ccatgatgtt cttaccaacc gattattgtt
       61 gcaggttaag cgatcaagag tatatggagc ttgtgtttga gaatggccag attcttgcaa
      121 agggccaaag atccaacgtt tctctgcata atcaacgtac caaatcgatc atggatttgt
      181 atgaggcaga gtataacgag gatttcatga agagtatcat ccatggtggt ggtggtgcca
      241 tcacaaatct cggggacacg caggttgttc cacaaagtca tgttgctgct gcccatgaaa
      301 caaacatgtt ggaaagcaat aaacatgttg actaagcggc cgctcgagca tgcatctaga
      361 gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct cgactgtgcc
      421 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      481 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      541 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      601 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      661 ctggggctct agggggtatc cccacgcgcc ctgtagcgg
//
