LOCUS       sequence-280961-        3002 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2435
FEATURES             Location/Qualifiers
     source          1..3002
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcagaact gtttatggaa tgtgaagaag aggagctgga accatggcag
       61 aagaaagtaa aagaagttga ggatgacgat gatgatgagc caatctttgt tggcgagata
      121 tcaagttcaa aaccagcaat ttcaaatatt ttgaacagag ttaaccccag ctcatattca
      181 aggggactaa agaatggtgc actcagtcga ggtattactg ctgcattcaa gcctacaagt
      241 caacactaca cgaatccaac atcaaatcca gtgcctgcct caccaataaa ttttcatcct
      301 gagtctagat cttcagatag ttctgttatt gttcagcctt tttctaaacc tggttatata
      361 acaaactcat cacgagttgt gtctaataag tcatcagagt tactgtttga cttgacccag
      421 gatacaggat tatcacatta ccaaggggga ccaacacttt ctatggcagg tatgagtgaa
      481 agttcatttt tatcaaaacg tccttctact tctgaagtaa ataatgttaa tccaaagaag
      541 cctaaaccca gcgaaagtgt ttctggagca aattcctcag ctgtattacc ttcagttaaa
      601 tctccttcag tgacttcttc ccaggctatg ctagcaaaag gtacaaatac ctcatcaaat
      661 cagtctaaaa atggaacacc ttttccaaga gcttgtccaa agtgcaacat tcatttcaat
      721 cttttggatc ctttgaaaaa tcacatgaag tattgttgtc cagacatgat aaataacttt
      781 ttgggactgg ctaaaacaga attttcaagt acagtaaata aaaacacaac tattgattca
      841 gagaaaggaa aattgatcat gttagttaat gacttttatt atggaaaaca tgaaggagat
      901 gtccaggagg aacagaagac tcacacaacc tttaaatgct tcagttgctt gaaaattcta
      961 aaaaataaca ttaggtttat gaaccacatg aaacaccatt tggaacttga gaagcagagc
     1021 agtgagagct gggaaaacca taccacctgc cagcactgct accgtcagtt tcccacacca
     1081 tttcagttgc agtgtcacat cgaaagtaca catacgcccc atgaattttc tactatttgt
     1141 aaaatctgtg aattgtcatt tgaaacagaa catgttcttt tacaacatat gaaggacaat
     1201 cataaacctg gtgaaatgcc atatgtctgc caggtttgta attatagatc atcatcattt
     1261 tctgatgtag aaactcattt tagaacatcc catgaaaaca ctaagaactt gctatgcccg
     1321 ttttgcctca aagttattaa aattgcaaca ccatatatgc atcattatat gaagcatcag
     1381 aaaaaaggaa tacatcgttg tacaaaatgc aggctgcagt ttttgacatg caaggagaaa
     1441 atggatcata agactcaaca ccatcgaaca tttataaaac ctaaacaact agaaggattg
     1501 cctcctggaa caaaagttac tattcgagct tcagttggac ctctgcaatc aggagcttca
     1561 cctacacctt ccattagtgc aagtgcttct acccttcagc tctcacctcc aaggactaaa
     1621 aatataactg ctaaaaatcc tgcaaaatct aatacaagta aacctaatac agtcaaatcc
     1681 aacgcaagta aacctaatac aagtaagcct aatggaagta aatctaaata caaaccaaaa
     1741 atctctaata tgcaaaagaa acaaagcaca ttggctagta gcaataaaaa aagtaaagtc
     1801 aatacagcat tgaggaattt aaggtatcgt cggggcattc acaaatgcat tgagtgttgt
     1861 tccgaaataa aagattttgc aaaccacttt cctacgtacg tccactgtag tttttgcaga
     1921 tacaacacta gctgtagcaa agcctatgta aatcatatga tgagctttca tagtaaccgt
     1981 ccaagcaaaa ggttttgtat ttttaagaag cattcagaaa atctccgggg cattactcta
     2041 gtgtgcctta attgtgattt cctaagtgat gtttctggct tagataatat ggctacacac
     2101 ttaagtcaac ataaaactca tacttgccaa gttgtaatgc agaaagtttc tgtttgtatc
     2161 ccaacttctg agcacctttc tgaattaaaa aaagaagctc ccgcaaagga acaagaacct
     2221 gtgtctaagg aaattgcaag acctaacatg gctgaaagag aaacagaaac atcaaattct
     2281 gaaagtaaac aagataaagc tgcttcttca aaagaaaaaa atggatgtaa tgcaaattca
     2341 tttgaaggct catcaacaac aaaaagtgaa gaaagcataa cagtttcaga taaggaaaat
     2401 gaaacctgtc ttgcagacca ggaaactggc tcaaaaaaca tcgtcagttg tgattcaaat
     2461 attggtgcag ataaagtgga aaagaaaaaa caaatacaac acgtttgtca ggaaatggag
     2521 ttgaagatgt gccaaagttc agaaaacata atcttatctg atcagattaa agatcacaac
     2581 tccagtgaag ccagattttc ttcaaagaat attaaggatt tgcgattagc atcagataat
     2641 gtaagcattg atcagttttt gagaaaaaga catgaacctg aatctgttag ttctgatgtt
     2701 agcgagcaag gcagtattca tttggaacct ctgactccat ccgaggtact tgagtatgaa
     2761 gccacagaga ttcttcagaa aggtagtggt gatccttcag ccaagactga tgaagtagtg
     2821 tctgatcaaa cagatgacat tcctggagga aataacccta gcacaacaga ggcaacagta
     2881 gacctggaag acgaaaaaga aagaagttag taatgaacta gtccgtaact tgaaagtatt
     2941 tcgatttctt ggctttatat atcttgtgga aaggacgatc aagatgcagt tacatcctat
     3001 gc
//
