LOCUS       sequence-280967-        3041 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2437
FEATURES             Location/Qualifiers
     source          1..3041
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggcgacaa cccttttcaa ccaaaaagta attcaaaaat ggcagaactg
       61 tttatggaat gtgaagaaga ggagctggaa ccatggcaga agaaagtaaa agaagttgag
      121 gatgacgatg atgatgagcc aatctttgtt ggcgagatat caagttcaaa accagcaatt
      181 tcaaatattt tgaacagagt taaccccagc tcatattcaa ggggactaaa gaatggtgca
      241 ctcagtcgag gtattactgc tgcattcaag cctacaagtc aacactacac gaatccaaca
      301 tcaaatccag tgcctgcctc accaataaat tttcatcctg agtctagatc ttcagatagt
      361 tctgttattg ttcagccttt ttctaaacct ggttatataa caaactcatc acgagttgtg
      421 tctaataagt catcagagtt actgtttgac ttgacccagg atacaggatt atcacattac
      481 caagggggac caacactttc tatggcaggt atgagtgaaa gttcattttt atcaaaacgt
      541 ccttctactt ctgaagtaaa taatgttaat ccaaagaagc ctaaacccag cgaaagtgtt
      601 tctggagcaa attcctcagc tgtattacct tcagttaaat ctccttcagt gacttcttcc
      661 caggctatgc tagcaaaagg tacaaatacc tcatcaaatc agtctaaaaa tggaacacct
      721 tttccaagag cttgtccaaa gtgcaacatt catttcaatc ttttggatcc tttgaaaaat
      781 cacatgaagt attgttgtcc agacatgata aataactttt tgggactggc taaaacagaa
      841 ttttcaagta cagtaaataa aaacacaact attgattcag agaaaggaaa attgatcatg
      901 ttagttaatg acttttatta tggaaaacat gaaggagatg tccaggagga acagaagact
      961 cacacaacct ttaaatgctt cagttgcttg aaaattctaa aaaataacat taggtttatg
     1021 aaccacatga aacaccattt ggaacttgag aagcagagca gtgagagctg ggaaaaccat
     1081 accacctgcc agcactgcta ccgtcagttt cccacaccat ttcagttgca gtgtcacatc
     1141 gaaagtacac atacgcccca tgaattttct actatttgta aaatctgtga attgtcattt
     1201 gaaacagaac atgttctttt acaacatatg aaggacaatc ataaacctgg tgaaatgcca
     1261 tatgtctgcc aggtttgtaa ttatagatca tcatcatttt ctgatgtaga aactcatttt
     1321 agaacatccc atgaaaacac taagaacttg ctatgcccgt tttgcctcaa agttattaaa
     1381 attgcaacac catatatgca tcattatatg aagcatcaga aaaaaggaat acatcgttgt
     1441 acaaaatgca ggctgcagtt tttgacatgc aaggagaaaa tggatcataa gactcaacac
     1501 catcgaacat ttataaaacc taaacaacta gaaggattgc ctcctggaac aaaagttact
     1561 attcgagctt cagttggacc tctgcaatca ggagcttcac ctacaccttc cattagtgca
     1621 agtgcttcta cccttcagct ctcacctcca aggactaaaa atataactgc taaaaatcct
     1681 gcaaaatcta atacaagtaa acctaataca gtcaaatcca acgcaagtaa acctaataca
     1741 agtaagccta atggaagtaa atctaaatac aaaccaaaaa tctctaatat gcaaaagaaa
     1801 caaagcacat tggctagtag caataaaaaa agtaaagtca atacagcatt gaggaattta
     1861 aggtatcgtc ggggcattca caaatgcatt gagtgttgtt ccgaaataaa agattttgca
     1921 aaccactttc ctacgtacgt ccactgtagt ttttgcagat acaacactag ctgtagcaaa
     1981 gcctatgtaa atcatatgat gagctttcat agtaaccgtc caagcaaaag gttttgtatt
     2041 tttaagaagc attcagaaaa tctccggggc attactctag tgtgccttaa ttgtgatttc
     2101 ctaagtgatg tttctggctt agataatatg gctacacact taagtcaaca taaaactcat
     2161 acttgccaag ttgtaatgca gaaagtttct gtttgtatcc caacttctga gcacctttct
     2221 gaattaaaaa aagaagctcc cgcaaaggaa caagaacctg tgtctaagga aattgcaaga
     2281 cctaacatgg ctgaaagaga aacagaaaca tcaaattctg aaagtaaaca agataaagct
     2341 gcttcttcaa aagaaaaaaa tggatgtaat gcaaattcat ttgaaggctc atcaacaaca
     2401 aaaagtgaag aaagcataac agtttcagat aaggaaaatg aaacctgtct tgcagaccag
     2461 gaaactggct caaaaaacat cgtcagttgt gattcaaata ttggtgcaga taaagtggaa
     2521 aagaaaaaac aaatacaaca cgtttgtcag gaaatggagt tgaagatgtg ccaaagttca
     2581 gaaaacataa tcttatctga tcagattaaa gatcacaact ccagtgaagc cagattttct
     2641 tcaaagaata ttaaggattt gcgattagca tcagataatg taagcattga tcagtttttg
     2701 agaaaaagac atgaacctga atctgttagt tctgatgtta gcgagcaagg cagtattcat
     2761 ttggaacctc tgactccatc cgaggtactt gagtatgaag ccacagagat tcttcagaaa
     2821 ggtagtggtg atccttcagc caagactgat gaagtagtgt ctgatcaaac agatgacatt
     2881 cctggaggaa ataaccctag cacaacagag gcaacagtag acctggaaga cgaaaaagaa
     2941 agaagttagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     3001 tcttgtggaa aggacgatcg tcagattcaa agggcccggt a
//
