LOCUS       sequence-280970-        1679 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1679)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2440
FEATURES             Location/Qualifiers
     source          1..1679
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             387..398
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggcctcaaa cgattatacc caacaagcaa cccaaagcta tggggcctac
       61 cccacccagc ccgggcaggg ctattcccag cagagcagtc agccctacgg acagcagagt
      121 tacagtggtt atagccagtc cacggacact tcaggctatg gccagagcag ctattcttct
      181 tatggccaga gccagaacag ctatggaact cagtcaactc cccagggata tggctcgact
      241 ggcggctatg gcagtagcca gagctcccaa tcgtcttacg ggcagcagtc ctcctaccct
      301 ggctatggcc agcagccagc tcccagcagc acctcgggaa gttacggtag cagttctcag
      361 agcagcagct atgggcagcc ccagagtggg agctacagcc agcagcctag ctatggtgga
      421 cagcagcaaa gctatggaca gcagcaaagc tataatcccc ctcagggcta tggacagcag
      481 aaccagtaca acagcagcag tggtggtgga ggtggaggtg gaggtggagg taactatggc
      541 caagatcaat cctccatgag tagtggtggt ggcagtggtg gcggttatgg caatcaagac
      601 cagagtggtg gaggtggcag cggtggctat ggacagcagg accgtggagg ccgcggcagg
      661 ggtggcagtg gtggcggcgg cggcggcggc ggtggtggtt acaaccgcag cagtggtggc
      721 tatgaaccca gaggtcgtgg aggtggccgt ggaggcagag gtggcatggg cggaagtgac
      781 cgtggtggct tcaataaatt tggtggccct cgggaccaag gatcacgtca tgactccgaa
      841 caggataatt cagacaacaa caccatcttt gtgcaaggcc tgggtgagaa tgttacaatt
      901 gagtctgtgg ctgattactt caagcagatt ggtattatta agacaaacaa gaaaacggga
      961 cagcccatga ttaatttgta cacagacagg gaaactggca agctgaaggg agaggcaacg
     1021 gtctcttttg atgacccacc ttcagctaaa gcagctattg actggtttga tggtaaagaa
     1081 ttctccggaa atcctatcaa ggtctcattt gctactcgcc gggcagactt taatcggggt
     1141 ggtggcaatg gtcgtggagg ccgagggcga ggaggaccca tgggccgtgg aggctatgga
     1201 ggtggtggca gtggtggtgg tggccgagga ggatttccca gtggaggtgg tggcggtgga
     1261 ggacagcagc gagctggtga ctggaagtgt cctaatccca cctgtgagaa tatgaacttc
     1321 tcttggagga atgaatgcaa ccagtgtaag gcccctaaac cagatggccc aggaggggga
     1381 ccaggtggct ctcacatggg gggtaactac ggggatgatc gtcgtggtgg cagaggaggc
     1441 tatgatcgag gcggctaccg gggccgcggc ggggaccgtg gaggcttccg agggggccgg
     1501 ggtggtgggg acagaggtgg ctttggccct ggcaagatgg attccagggg tgagcacaga
     1561 caggatcgca gggagaggcc gtattagtaa tgaactagtc cgtaacttga aagtatttcg
     1621 atttcttggc tttatatatc ttgtggaaag gacgagacgc tcatgccgtc tctctgcga
//
