LOCUS       sequence-280979-        1295 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1295)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1295)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2479
FEATURES             Location/Qualifiers
     source          1..1295
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccagtc gcctcctccg cagagcctcc tcggccacga ccactggatc
       61 ttcgcccagg gctggggctg ggccggccac tgggactcca cgtccccggc ctcctcctcc
      121 gattcgtcgg gttcgtgccc ctgcgacggc gcccgcggac tcccgcagcc acagcctccg
      181 agctgcagct cccgagccgc agaggcagcc gcgacgacgc ccagacgagc gcgcaccgga
      241 ccagcgggcg gacagcggca gagcgccagc gagcgggaga aactgcgcat gcgcacgctg
      301 gcccgcgccc tgcacgagtt gcgccgcttt ctgcctccct ccttggcgcc ggccggccag
      361 agcctgacca agatcgagac gctgcgcctg gccatccgct acatcggcca cctatcggcc
      421 gtgctgggtc tcagcgagga gagtctgcag tgccggcgca ggcagcgcgg ggacgcgggg
      481 tccccttggg gctgcccgct gtgccccgac cgtggccccg cagaggcgca gacgcaggcg
      541 gaggggcagg ggcaagggca ggggcagggg caggggcaag ggcaggggca aggacagggg
      601 caaggacagg ggcaagggca ggggcgcagg ccgggcctgg tctccgccgt cctcgccgag
      661 gcgtcctggg gatccccgtc cgcctgcccc ggagcccaag ccgcacccga gcgcctgggg
      721 aggggggtcc acgacacgga tccctgggca acaccccctt actgccccaa gatacagtcg
      781 cccccgtatt cgtcccaagg gacaacctcc gacgcgtctc tttggacgcc accccaaggc
      841 tgtccctgga cgcagtcgtc cccagagccc cggaacccac cagtgccctg gacggcggcc
      901 ccagcaactt tggagctggc cgcagtgtac cagggtctct ctgtgtctcc agagccctgt
      961 ctgtcgctgg gagctccatc tctcctgccc cacccatcat gccagagact gcagcctcag
     1021 acccccggga ggtgctggag ccacagtgca gaggtggtgc ccaactcaga ggaccaggga
     1081 ccgggcgccg ccttccagct cagtgaagca agccctcccc agagctcagg cctgcggttc
     1141 agtggctgcc ctgaactttg gcaagaagat ctggaggggg cccgcctggg catcttctac
     1201 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1261 ggaaaggacg atccgacaga aggccctagg cgcat
//
