LOCUS       sequence-28098-e         580 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Tim DH-PH domains fused to mYFP-PIF6, for optogenetic 
            recruitment.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Levskaya A, Weiner OD, Lim WA, Voigt CA
  TITLE     Spatiotemporal control of cell signalling using a light-switchable 
            protein interaction.
  JOURNAL   Nature. 2009 Oct 15;461(7266):997-1001.
  PUBMED    19749742
REFERENCE   2  (bases 1 to 580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature."; 
            date: "2009-10-15"; volume: "461(7266)"; pages: "997-1001"
COMMENT     Sequence Label: pAL190
FEATURES             Location/Qualifiers
     source          1..580
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(368..386)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(369..386)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(406..423)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    412..523
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctcggcatgg acgagctgta caagggatcc atgatgttct taccaaccga ttattgttgc
       61 aggttaagcg atcaagagta tatggagctt gtgtttgaga atggccagat tcttgcaaag
      121 ggccaaagat ccaacgtttc tctgcataat caacgtacca aatcgatcat ggatttgtat
      181 gaggcagagt ataacgagga tttcatgaag agtatcatcc atggtggtgg tggtgccatc
      241 acaaatctcg gggacacgca ggttgttcca caaagtcatg ttgctgctgc ccatgaaaca
      301 aacatgttgg aaagcaataa acatgttgac taagcggccg ctcgagcatg catctagagg
      361 gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg actgtgcctt
      421 ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg
      481 ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt
      541 gtcattctat tctggggggt ggggtggggc aggacagcaa
//
