LOCUS       sequence-280982-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2481
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagctgaa ctccctgctg atcctgctgg aggcggccga gtacctggag
       61 cgcagggatc gagaggccga gcacggctac gcctcggtgc tgcccttcga cggcgacttc
      121 gccagggaga aaacaaaggc ggccggcctg gtgcgcaagg ccccgaacaa caggtcttca
      181 cacaacgagc tagaaaagca cagacgagcc aaactcaggc tgtaccttga gcagctcaag
      241 caactggtgc ccctgggccc cgacagcacc cgccacacca cgctgagcct cctgaagcgg
      301 gccaaggtgc acatcaagaa actggaggag caggaccgcc gggcactgag catcaaggag
      361 cagctgcagc aggagcatcg tttcctgaag cggcgcctgg agcagctgtc ggtgcagagc
      421 gtggagcgcg tgcgcacaga tagcacgggc tctgctgtct ccacggacga ctcagagcaa
      481 gaagtggaca tagagggcat ggagtttggc cctggtgagc tggacagtgt tggcagcagc
      541 agtgacgcgg acgaccacta cagcctgcag agtggcaccg gcggcgacag tggcttcggg
      601 ccccactgcc ggcggctggg ccgccccgcc ctctcgtagt aatgaactag tccgtaactt
      661 gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgacgc acactataat
      721 cagtctgaag g
//
