LOCUS       sequence-281006-        2516 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2535
FEATURES             Location/Qualifiers
     source          1..2516
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggaagaat gtcttccaag caagccacct ctccatttgc ctgtgcagct
       61 gatggagagg atgcaatgac ccaggattta acctcaaggg aaaaggaaga gggcagtgat
      121 caacatgtgg cctcccatct gcctctgcac cccataatgc acaacaaacc tcactctgag
      181 gagctaccaa cacttgtcag taccattcaa caagatgctg actgggacag cgttctgtca
      241 tctcagcaaa gaatggaatc agagaataat aagttatgtt ccctatattc cttccgaaat
      301 acctctacct caccacataa gcctgacgaa gggagtcggg accgtgagat aatgaccagt
      361 gttacttttg gaaccccaga gcgccgcaaa gggagtcttg ccgatgtggt ggacacactg
      421 aaacagaaga agcttgagga aatgactcgg actgaacaag aggattcctc ctgcatggaa
      481 aaactacttt caaaagattg gaaggaaaaa atggaaagac taaataccag tgaacttctt
      541 ggagaaatta aaggtacacc tgagagcctg gcagaaaaag aacggcagct ctccaccatg
      601 attacccagc tgatcagttt acgggagcag ctactggcag cgcatgatga acagaaaaaa
      661 ctggcagcgt cacaaattga gaaacaacgg cagcaaatgg accttgctcg ccaacagcaa
      721 gaacagattg cgagacaaca gcagcaactt ctgcaacagc agcacaaaat taatctcctg
      781 cagcaacaga tccaggttca gggtcacatg cctccgctca tgatcccaat ttttccacat
      841 gaccagcgga ctctggcagc agctgctgct gcccaacagg gattcctctt cccccctgga
      901 ataacataca aaccaggtga taactacccc gtacagttca ttccatcaac aatggcagct
      961 gctgctgctt ctggactcag ccctttacag ctccagcagc tctatgccgc tcagctggcc
     1021 agcatgcagg tgtcacctgg agcaaagatg ccatcaactc cacagccacc aaacacagca
     1081 gggacggtct cacctactgg gataaaaaat gaaaagagag ggaccagccc tgtaactcaa
     1141 gttaaggatg aagcagcagc acagcctctg aatctctcat cccgacccaa gacagcagag
     1201 cctgtaaagt ccccaacgtc tcccacccag aacctcttcc cagccagcaa aaccagccct
     1261 gtcaatctgc caaacaaaag cagcatccct agccccattg gaggaagcct gggaagagga
     1321 tcctctttag gtaaatggaa aagtcaacac caggaagaga cttacgaatt agatatccta
     1381 tctagtctca actcccctgc cctttttggg gatcaggata cagtgatgaa agccattcag
     1441 gaggcgcgga agatgcgaga gcagatccag cgggagcaac agcagcaaca gccacatggt
     1501 gttgacggga aactgtcctc cataaataat atggggctga acagctgcag gaatgaaaag
     1561 gaaagaacgc gctttgagaa tttggggccc cagttaacgg gaaagtcaaa tgaagatgga
     1621 aaactgggcc caggtgtcat cgaccttact cggccagaag atgcagaggg aagtaaagca
     1681 atgaatggct ctgcagctaa actacagcag tattattgtt ggccaacagg aggtgccact
     1741 gtggctgaag cacgagtcta cagggacgcc cgcggccgtg ccagcagcga gccacacatt
     1801 aagcgaccaa tgaatgcatt catggtttgg gcaaaggatg agaggagaaa aatccttcag
     1861 gccttccccg acatgcataa ctccaacatt agcaaaatct taggatctcg ctggaaatca
     1921 atgtccaacc aggagaagca accttattat gaagagcagg cccggctaag caagatccac
     1981 ttagagaagt acccaaacta taaatacaaa ccccgaccga aacgcacctg cattgttgat
     2041 ggcaaaaagc ttcggattgg ggagtataag caactgatga ggtctcggag acaggagatg
     2101 aggcagttct ttactgtggg gcaacagcct cagattccaa tcaccacagg aacaggtgtt
     2161 gtgtatcctg gtgctatcac tatggcaact accacaccat cgcctcagat gacatctgac
     2221 tgctctagca cctcggccag cccggagccc agcctcccgg tcatccagag cacttatggt
     2281 atgaagacag atggcggaag cctagctgga aatgaaatga tcaatggaga ggatgaaatg
     2341 gaaatgtatg atgactatga agatgacccc aaatcagact atagcagtga aaatgaagcc
     2401 ccggaggctg tcagtgccaa ctagtaatga actagtccgt aacttgaaag tatttcgatt
     2461 tcttggcttt atatatcttg tggaaaggac gacccagagc ctctccacag atagac
//
