LOCUS       sequence-281021-        1178 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2580
FEATURES             Location/Qualifiers
     source          1..1178
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccggag gtatgatgag ctgccgcact acccaggcat cgtggatggc
       61 cccgcagccc tggctagctt cccagagaca gtgcccgcag taccagggcc ctatggcccg
      121 caccggcctc cccagcccct gcccccaggc ttggacagcg acggcctgaa gagggagaag
      181 gatgagatct atggacaccc gctcttcccc ctcttggccc tggtctttga gaaatgtgaa
      241 ctggctacat gctctccccg tgacggggcc ggagctgggc tggggacacc ccctggaggt
      301 gacgtctgct cctctgattc cttcaacgag gacatcgctg cctttgccaa gcaggttcgc
      361 tctgagaggc ccctcttctc ctccaaccca gaactggaca atctgatgat ccaggccatc
      421 caggtgctgc ggttccacct gctggagctg gagaagggaa agatgcccat cgacctggtc
      481 atcgaggatc gggacggcgg ctgcagggag gacttcgagg actacccagc ctcctgcccc
      541 agcctcccag accagaataa tatgtggatt cgagaccatg aggatagtgg gtctgtacat
      601 ttggggaccc caggtccatc cagtgggggc ctggcctccc agagtgggga caactccagt
      661 gaccaaggag acgggctgga caccagcgtg gcctctccca gttctggtgg agaagatgag
      721 gacttggacc aggagcgacg gcgaaacaag aagaggggga tcttccccaa ggtggccacc
      781 aacatcatgc gagcctggtt gttccagcac ctctcgcacc cgtacccctc ggaggagcag
      841 aagaaacagc tggcgcagga cacggggctc accatcctgc aagtcaacaa ctggttcatt
      901 aacgcccgga gacgcatcgt gcaacctatg atcgatcaat ccaaccgcac agggcagggt
      961 gcagccttca gcccagaggg ccagcccatc gggggctata ccgagacgca gccacacgtg
     1021 gccgtccggc ctccgggatc agtggggatg agtttgaact tggaaggaga atggcattat
     1081 ctatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1141 tgtggaaagg acgaaatcgt cgaggttctc ctcaaagg
//
