LOCUS       sequence-281030-        1937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1937)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2602
FEATURES             Location/Qualifiers
     source          1..1937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggatcctgg agccgggtca gagacatctc tgactgtcaa tgagcaggtc
       61 atcgtgatgt caggtcatga gaccatccga gtgctggaag tcggagtgga tgcccaactc
      121 cctgctgagg aagagagcaa aggactggag ggtgtggccg ccgagggctc ccagagcgga
      181 gaccctgctg aagccagtca agctgctggt gaagctgggc cagacaacct gggctcctct
      241 gcagaggcaa ctgtgaagtc acccccgggg atccctccga gccctgccac tgccattgcc
      301 accttcagcc aagccccaag ccagcctcag gcatcgcaga ccctgacgcc actggctgta
      361 caagctgccc cccaggtctt gactcaggaa aacttagcca cagttctgac aggagttatg
      421 gttccagcag gggcagttac tcaacctctt cttatcccca tcagtattgc aggtcaagtg
      481 gctggtcagc aggggctggc cgtgtggaca attcctacag caactgtggc tgccctccca
      541 ggactgaccg ctgcttctcc tacgggggga gtgttcaagc cacctttagc cggtctccaa
      601 gcagctgctg tgctgaacac cgctcttccg gcaccggtac aagctgccgc accagtacag
      661 gcctcctcga cggcccaacc ccggccacca gcccagcccc agacgctgtt ccagacccag
      721 ccgctgctgc agaccacacc tgccatcctc ccgcagccca ctgctgccac cgctgctgcc
      781 cctaccccca agccagtgga caccccccca cagatcaccg tccagcctgc aggcttcgca
      841 tttagcccag gaatcatcag tgctgcttcc ctcgggggac agacccagat cctggggtcc
      901 ctcactacag ctccagtcat taccagcgcc attcccagca tgccagggat cagcagtcag
      961 atcctcacca atgctcaggg acaggttatt ggaacccttc catgggtagt gaactcagct
     1021 agtgtggcgg ccccagcacc agcccaaagc ctgcaggtcc aggccgtgac cccccagctg
     1081 ttgttgaacg cccagggcca ggtgattgcg accctggcta gcagccccct gcctccacct
     1141 gtggctgtcc ggaagccaag cacacctgag tcccctgcta agagtgaggt gcagcccatc
     1201 cagcccacac caaccgtgcc ccagcctgct gtggtcattg ccagcccagc tccagccgcc
     1261 aagccatctg cctctgctcc tatcccaatt acctgctcag agacccccac cgtcagccag
     1321 ttggtgtcca agccacatac tccaagtctg gatgaggatg ggatcaactt agaagagatc
     1381 cgggagtttg ccaagaactt taagatccgg cggctctcgc tgggccttac acagacccag
     1441 gtgggtcagg ctctgactgc aacggaaggt ccagcctaca gccagtcagc catctgccgg
     1501 ttcgagaagc tagacatcac acccaagagt gcccagaagc taaagccggt gctggaaaag
     1561 tggctaaacg aagctgaact gcggaaccag gaaggccagc agaacctgat ggagtttgtg
     1621 ggaggcgagc cctccaagaa acgcaaacgc cgcacctcct tcacccccca ggccatagag
     1681 gctctcaatg cctattttga gaagaaccca ctgcccacag gccaggagat cactgaaatt
     1741 gctaaggagc tcaactacga ccgtgaggta gtgcgggtct ggttctgcaa tcggcgccag
     1801 acgctcaaga acaccagcaa gctgaacgtc tttcagatcc cttagtaatg aactagtccg
     1861 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgactgcctc
     1921 aactggtgct tagcgca
//
