LOCUS       sequence-281033-        1454 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2611
FEATURES             Location/Qualifiers
     source          1..1454
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             63..74
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggctgcttc caagacccag ggggctgtcg cccgaatgca ggaagaccgt
       61 gatgggagct gcagcacagt cgggggtgta ggttatgggg acagtaagga ttgtatcctg
      121 gagccgcttt ccctgccaga aagtccaggt ggcaccacca ctttagaagg ttctccatct
      181 gtgccttgta ttttctgtga agaacatttt cctgtggctg aacaagacaa acttctgaag
      241 cacatgatta ttgagcataa gattgtcata gctgatgtca agttggttgc tgatttccaa
      301 aggtacattt tatattggag gaaaaggttc actgaacagc ccatcacaga tttttgtagt
      361 gtaataagaa ttaattccac tgctccattt gaagaacaag agaattattt tttgttatgt
      421 gacgttttac cagaagatag aattcttaga gaagagcttc agaaacagag actgagagaa
      481 attctggaac aacagcagca agaacgaaat gataccaatt ttcatggcgt ttgtatgttt
      541 tgcaatgaag aattccttgg aaacagatct gttattttga accacatggc cagagaacat
      601 gctttcaaca ttggattgcc agacaacatt gtaaactgca atgaattttt gtgtacatta
      661 cagaaaaagc ttgacaattt gcagtgcttg tactgtgaga agaccttcag ggacaaaaat
      721 acacttaaag atcacatgag gaaaaaacag catcgtaaga ttaatcctaa gaacagagaa
      781 tatgacagat tttatgtcat caattatttg gaacttggaa aatcgtggga agaagttcag
      841 ttggaagatg atcgggagtt gctggaccat caggaagatg actggtctga ttgggaagaa
      901 caccctgcct ctgcagtctg cttattttgt gaaaagcaag cagaaacaat tgagaagttg
      961 tatgtccaca tggaggatgc acacgaattt gatcttctca aaataaagtc agaacttgga
     1021 ttaaatttct atcagcaagt gaaactggtc aattttattc ggaggcaagt tcaccaatgc
     1081 agatgttatg gctgccatgt gaagttcaaa tccaaagcag acttaagaac tcacatggaa
     1141 gaaactaaac acacttcgct gctccccgat agaaagacgt gggatcaact ggagtattat
     1201 tttccaacct atgaaaatga cactctcctg tgtacactat ctgacagtga aagtgacctg
     1261 acagctcagg aacaaaatga aaatgttccc atcatcagtg aagatacatc taaactgtat
     1321 gctttgaaac aaagcagtat tttgaaccag ttgctactat agtaatgaac tagtccgtaa
     1381 cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga caaagatctt
     1441 gcgaggagta caat
//
