LOCUS       sequence-281045-        2012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2652
FEATURES             Location/Qualifiers
     source          1..2012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      8..17
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcggcgga agaggaggct gcggcgggag gtaaagtgtt gagagaggag
       61 aaccagtgca ttgctcctgt ggtttccagc cgcgtgagtc cagggacaag accaacagct
      121 atggggtctt tcagctcaca catgacagag tttccacgaa aacgcaaagg aagtgattca
      181 gacccatccc agtcaggaat catgacagaa aaagtggtgg aaaagctttc tcagaatccc
      241 cttacctatc ttctttcaac aaggatagaa atatcagcct ccagtggcag cagagtggaa
      301 gatggtgaac accaagttaa aatgaaggcc ttcagagaag ctcatagcca aactgaaaag
      361 cggaggagag ataaaatgaa taacctgatt gaagaactgt ctgcaatgat ccctcagtgc
      421 aaccccatgg cgcgtaaact ggacaaactt acagttttaa gaatggctgt tcaacacttg
      481 agatctttaa aaggcttgac aaattcttat gtgggaagta attatagacc atcatttctt
      541 caggataatg agctcagaca tttaatcctt aagactgcag aaggcttctt atttgtggtt
      601 ggatgtgaaa gaggaaaaat tctcttcgtt tctaagtcag tctccaaaat acttaattat
      661 gatcaggcta gtttgactgg acaaagctta tttgacttct tacatccaaa agatgttgcc
      721 aaagtaaagg aacaactttc ttcttttgat atttcaccaa gagaaaagct aatagatgcc
      781 aaaactggtt tgcaagttca cagtaatctc cacgctggaa ggacacgtgt gtattctggc
      841 tcaagacgat cttttttctg tcggataaag agttgtaaaa tctctgtcaa agaagagcat
      901 ggatgcttac ccaactcaaa gaagaaagag cacagaaaat tctatactat ccattgcact
      961 ggttacttga gaagctggcc tccaaatatt gttggaatgg aagaagaaag gaacagtaag
     1021 aaagacaaca gtaattttac ctgccttgtg gccattggaa gattacagcc atatattgtt
     1081 ccacagaaca gtggagagat taatgtgaaa ccaactgaat ttataacccg gtttgcagtg
     1141 aatggaaaat ttgtctatgt agatcaaagg gcaacagcga ttttaggata tctgcctcag
     1201 gaacttttgg gaacttcttg ttatgaatat tttcatcaag atgaccacaa taatttgact
     1261 gacaagcaca aagcagttct acagagtaag gagaaaatac ttacagattc ctacaaattc
     1321 agagcaaaag atggctcttt tgtaacttta aaaagccaat ggtttagttt cacaaatcct
     1381 tggacaaaag aactggaata tattgtatct gtcaacactt tagttttggg acatagtgag
     1441 cctggagaag catcattttt accttgtagc tctcaatcat cagaagaatc ctctagacag
     1501 tcctgtatga gtgtacctgg aatgtctact ggaacagtac ttggtgctgg tagtattgga
     1561 acagatattg caaatgaaat tctggattta cagaggttac agtcttcttc ataccttgat
     1621 gattcgagtc caacaggttt aatgaaagat actcatactg taaactgcag gagtatgtca
     1681 aataaggagt tgtttccacc aagtccttct gaaatggggg agctagaggc taccaggcaa
     1741 aaccagagta ctgttgctgt ccacagccat gagccactcc tcagtgatgg tgcacagttg
     1801 gatttcgatg ccctatgtga caatgatgac acagccatgg ctgcatttat gaattactta
     1861 gaagcagagg ggggcctggg agaccctggg gacttcagtg acatccagtg gaccctctag
     1921 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1981 aaggacgata gacggagtac gtcgcccgta cg
//
