LOCUS       sequence-28105-e         937 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 937)
  AUTHORS   Rosenthal JA, Chen H, Slepnev VI, Pellegrini L, Salcini AE, Di Fiore
            PP, De Camilli P
  TITLE     The epsins define a family of proteins that interact with components
            of the clathrin coat and contain a new protein module.
  JOURNAL   J Biol Chem. 1999 Nov 26. 274(48):33959-65.
  PUBMED    10567358
REFERENCE   2  (bases 1 to 937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 1999 Nov 26. 274(48):33959-65."
COMMENT     Sequence Label: pcDNA3-HA-Epsin 2
FEATURES             Location/Qualifiers
     source          1..937
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     63..82
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        63..81
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             107..133
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 cggggggntt tagcganctc tctggctact agagaaccca ctgcttactg gcttatcgaa
       61 attaatacga ctcactatag ggagacccaa gcttggtacc atggcgtacc catacgacgt
      121 cccagactac gcgggatctc gagctcaagc ttcgaattct atgacaactt cgtctatcag
      181 acggcagatg aaaaacatcg tgaacaatta ctcagaggca gaaatcaaag tccgggaagc
      241 cacctccaat gacccgtggg gcccgtccag ttctctgatg accgagattg ccgacctgac
      301 ctacaacgtg gtggccttct cggagatcat gagcatggtg tggaagcggc tgaatgacca
      361 tggcaagaac tggcggcatg tgtacaaggc gctgaccctg ctggactacc tcatcaagac
      421 aggctccgaa cgtgtggccc agcagtgccg ggagaacatc ttcgccatcc agaccctgaa
      481 ggacttccag tacattgacc gagatggcaa ggaccagggc atcaatgtgc gtgagaagtc
      541 aaagcaactg gtggctctcc tcaaggacga ggaacggttg aaggctgaga gggcccaggc
      601 tctcaaaacc aaagagcgca tggcccaggt tgccactggc atgggcagca accagatcac
      661 ctttgggcga ggctccagcc agcccaacct ctccaccagc cactcggagc aggagtatgg
      721 caaggccggg ggctccccgg cctcctacca tggctcgcct gaggcctcgc tgtgccccca
      781 gcaccgcaca ggggccccgc tgggtcagag tgaggagctg cagccactga gccagcgcca
      841 ccccttcctg ccgcacctgg ggctggcctc ccgcccaaaa tggcgactgg tcccagccct
      901 gcctcacttg tgaccgcgca gcccgagcca cctcccc
//
