LOCUS       sequence-281057-        1655 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1655)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1655)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2683
FEATURES             Location/Qualifiers
     source          1..1655
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcacgttt aacaaaaaga cgacaggcgg atacaaaagc tatccagcat
       61 ctttgggcag ccattgagat tatacggaac cagaagcaga ttgccaacat tgaccgtatt
      121 acaaagtata tgtctcgagt ccacggtatg caccctaaag agaccacccg tcagctgagc
      181 ttagctgtga aagatggtct tattgtcgaa actctaacag tgggctgcaa aggttcaaaa
      241 gctggtattg aacaagaagg atattggttg ccaggagatg agattagcat taagaagaag
      301 aatacaaaca aacaggagat gggcacatac ctcagattca ttgtctcccg catgaaggag
      361 aggaaagtga atgaagggaa ataccgaagt tatgaagagt tcaaagctga tgcccaattg
      421 cttctccaca ataccgtgat tttctatgga gcagacagtg agcaagctga cattgcgagg
      481 atgctatata aagacacatg tcatgagctg gatgaactgc agctttgcaa gaattgcttt
      541 tacttgtcaa atgctcgtcc tgacaactgg ttctgttatc cttgtatacc taatcatgag
      601 ctggtttggg ctaaaatgaa aggttttggg ttttggccag ccaaagtcat gcagaaagaa
      661 gacaatcaag tcgacgttcg cttctttggc caccaccacc agagggcctg gattccttct
      721 gaaaacattc aagatatcac agtcaacatt catcggctgc acgtgaagcg cagtatgggt
      781 tggaaaaagg cctgtgatga gctggagctg catcagcgtt tcctacgaga agggagattt
      841 tggaaatcta agaatgagga ccgaggtgag gaagaggcag aatccagtat ctcctccacc
      901 agtaatgagc agctaaaggt cactcaagaa ccaagagcaa agaaaggacg acgtaatcaa
      961 agtgtggagc ccaaaaagga agaaccagag cctgaaacag aagcagtaag ttctagccag
     1021 gaaataccca cgatgcctca gcccatcgaa aaagtctccg tgtcaactca gacaaagaag
     1081 ttaagtgcct cttcaccaag aatgctgcat cggagcaccc agaccacaaa cgacggcgtg
     1141 tgtcagagca tgtgccatga caaatacacc aagatcttca atgacttcaa agaccggatg
     1201 aagtcggacc acaagcggga gacagagcgt gttgtccgag aagctctgga gaagctgcgt
     1261 tctgaaatgg aagaagaaaa gagacaagct gtaaataaag ctgtagccaa catgcagggt
     1321 gagatggaca gaaaatgtaa gcaagtaaag gaaaagtgta aggaagaatt tgtagaagaa
     1381 atcaagaagc tggcaacaca gcacaagcaa ctgatttctc agaccaagaa gaagcagtgg
     1441 tgctacaact gtgaggagga ggccatgtac cactgctgct ggaacacatc ctactgctcc
     1501 atcaagtgcc agcaggagca ctggcacgcg gagcacaagc gcacctgccg ccggaaaaga
     1561 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     1621 ggaaaggacg atcttccgtc catccctagc tacct
//
