LOCUS       sequence-281060-        1859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2685
FEATURES             Location/Qualifiers
     source          1..1859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgatttt tacatcatta cacagatatg tcattttcat tagttgtatc
       61 attgttataa actggtatat gtctcgagtc cacggtatgc accctaaaga gaccacccgt
      121 cagctgagct tagctgtgaa agatggtctt attgtcgaaa ctctaacagt gggctgcaaa
      181 ggttcaaaag ctggtattga acaagaagga tattggttgc caggagatga gattgactgg
      241 gaaacagaaa atcatgactg gtattgtttt gaatgccatt tgcctggaga ggtgttgata
      301 tgtgacctgt gttttcgtgt gtatcattcc aagtgtttgt ctgatgagtt caggcttaga
      361 gacagcagta gtccctggca gtgcccagtt tgcaggagca ttaagaagaa gaatacaaac
      421 aaacaggaga tgggcacata cctcagattc attgtctccc gcatgaagga gagggctata
      481 gatcttaata aaaaggggaa ggacaataaa cacccgatgt acaggaggct ggtgcactca
      541 gctgtggacg ttcccaccat tcaagagaaa gtgaatgaag ggaaataccg aagttatgaa
      601 gagttcaaag ctgatgccca attgcttctc cacaataccg tgattttcta tggagcagac
      661 agtgagcaag ctgacattgc gaggatgcta tataaagaca catgtcatga gctggatgaa
      721 ctgcagcttt gcaagaattg cttttacttg tcaaatgctc gtcctgacaa ctggttctgt
      781 tatccttgta tacctaatca tgagctggtt tgggctaaaa tgaaaggttt tgggttttgg
      841 ccagccaaag tcatgcagaa agaagacaat caagtcgacg ttcgcttctt tggccaccac
      901 caccagaggg cctggattcc ttctgaaaac attcaagata tcacagtcaa cattcatcgg
      961 ctgcacgtga agcgcagtat gggttggaaa aaggcctgtg atgagctgga gctgcatcag
     1021 cgtttcctac gagaagggag attttggaaa tctaagaatg aggaccgagg tgaggaagag
     1081 gcagaatcca gtatctcctc caccagtaat gagcagctaa aggtcactca agaaccaaga
     1141 gcaaagaaag gacgacgtaa tcaaagtgtg gagcccaaaa aggaagaacc agagcctgaa
     1201 acagaagcag taagttctag ccaggaaata cccacgatgc ctcagcccat cgaaaaagtc
     1261 tccgtgtcaa ctcagacaaa gaagttaagt gcctcttcac caagaatgct gcatcggagc
     1321 acccagacca caaacgacgg cgtgtgtcag agcatgtgcc atgacaaata caccaagatc
     1381 ttcaatgact tcaaagaccg gatgaagtcg gaccacaagc gggagacaga gcgtgttgtc
     1441 cgagaagctc tggagaagct gcgttctgaa atggaagaag aaaagagaca agctgtaaat
     1501 aaagctgtag ccaacatgca gggtgagatg gacagaaaat gtaagcaagt aaaggaaaag
     1561 tgtaaggaag aatttgtaga agaaatcaag aagctggcaa cacagcacaa gcaactgatt
     1621 tctcagacca agaagaagca gtggtgctac aactgtgagg aggaggccat gtaccactgc
     1681 tgctggaaca catcctactg ctccatcaag tgccagcagg agcactggca cgcggagcac
     1741 aagcgcacct gccgccggaa aagatagtaa tgaactagtc cgtaacttga aagtatttcg
     1801 atttcttggc tttatatatc ttgtggaaag gacgaaatct ctctagcgta agctttctc
//
