LOCUS       sequence-281072-        1478 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1478)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1478)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2700
FEATURES             Location/Qualifiers
     source          1..1478
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             204..215
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tggattcggt agaactttgc cttcctgaat ctttttccct gcactacgag
       61 gaagagcttc tctgcagaat gtcaaacaaa gatcgacaca ttgattccag ctgttcgtcc
      121 ttcatcaaga cggaaccttc cagcccagcc tccctgacgg acagcgtcaa ccaccacagc
      181 cctggtggct cttcagacgc cagtgggagc tacagttcaa ccatgaatgg ccatcagaac
      241 ggacttgact cgccacctct ctacccttct gctcctatcc tgggaggtag tgggcctgtc
      301 aggaaactgt atgatgactg ctccagcacc attgttgaag atccccagac caagtgtgaa
      361 tacatgctca actcgatgcc caagagactg tgtttagtgt gtggtgacat cgcttctggg
      421 taccactatg gggtagcatc atgtgaagcc tgcaaggcat tcttcaagag gacaattcaa
      481 ggcaatatag aatacagctg ccctgccacg aatgaatgtg aaatcacaaa gcgcagacgt
      541 aaatcctgcc aggcttgccg cttcatgaag tgtttaaaag tgggcatgct gaaagaaggg
      601 gtgcgtcttg acagagtacg tggaggtcgg cagaagtaca agcgcaggat agatgcggag
      661 aacagcccat acctgaaccc tcagctggtt cagccagcca aaaagccata taacaagatt
      721 gtctcacatt tgttggtggc tgaaccggag aagatctatg ccatgcctga ccctactgtc
      781 cccgacagtg acatcaaagc cctcactaca ctgtgtgact tggccgaccg agagttggtg
      841 gttatcattg gatgggcgaa gcatattcca ggcttctcca cgctgtccct ggcggaccag
      901 atgagccttc tgcagagtgc ttggatggaa attttgatcc ttggtgtcgt ataccggtct
      961 ctttcgtttg aggatgaact tgtctatgca gacgattata taatggacga agaccagtcc
     1021 aaattagcag gccttcttga tctaaataat gctatcctgc agctggtaaa gaaatacaag
     1081 agcatgaagc tggaaaaaga agaatttgtc accctcaaag ctatagctct tgctaattca
     1141 gactccatgc acatagaaga tgttgaagcc gttcagaagc ttcaggatgt cttacatgaa
     1201 gcgctgcagg attatgaagc tggccagcac atggaagacc ctcgtcgagc tggcaagatg
     1261 ctgatgacac tgccactcct gaggcagacc tctaccaagg ccgtgcagca tttctacaac
     1321 atcaaactag aaggcaaagt cccaatgcac aaactttttt tggaaatgtt ggaggccaag
     1381 gtctagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct ttatatatct
     1441 tgtggaaagg acgattggaa cacttgatag ggatagaa
//
