LOCUS       sequence-281075-        1292 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1292)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2702
FEATURES             Location/Qualifiers
     source          1..1292
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             135..146
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 accgccacca tgtcaaacaa agatcgacac attgattcca gctgttcgtc cttcatcaag
       61 acggaacctt ccagcccagc ctccctgacg gacagcgtca accaccacag ccctggtggc
      121 tcttcagacg ccagtgggag ctacagttca accatgaatg gccatcagaa cggacttgac
      181 tcgccacctc tctacccttc tgctcctatc ctgggaggta gtgggcctgt caggaaactg
      241 tatgatgact gctccagcac cattgttgaa gatccccaga ccaagtgtga atacatgctc
      301 aactcgatgc ccaagagact gtgtttagtg tgtggtgaca tcgcttctgg gtaccactat
      361 ggggtagcat catgtgaagc ctgcaaggca ttcttcaaga ggacaattca aggggtgcgt
      421 cttgacagag tacgtggagg tcggcagaag tacaagcgca ggatagatgc ggagaacagc
      481 ccatacctga accctcagct ggttcagcca gccaaaaagc catataacaa gattgtctca
      541 catttgttgg tggctgaacc ggagaagatc tatgccatgc ctgaccctac tgtccccgac
      601 agtgacatca aagccctcac tacactgtgt gacttggccg accgagagtt ggtggttatc
      661 attggatggg cgaagcatat tccaggcttc tccacgctgt ccctggcgga ccagatgagc
      721 cttctgcaga gtgcttggat ggaaattttg atccttggtg tcgtataccg gtctctttcg
      781 tttgaggatg aacttgtcta tgcagacgat tatataatgg acgaagacca gtccaaatta
      841 gcaggccttc ttgatctaaa taatgctatc ctgcagctgg taaagaaata caagagcatg
      901 aagctggaaa aagaagaatt tgtcaccctc aaagctatag ctcttgctaa ttcagactcc
      961 atgcacatag aagatgttga agccgttcag aagcttcagg atgtcttaca tgaagcgctg
     1021 caggattatg aagctggcca gcacatggaa gaccctcgtc gagctggcaa gatgctgatg
     1081 acactgccac tcctgaggca gacctctacc aaggccgtgc agcatttcta caacatcaaa
     1141 ctagaaggca aagtcccaat gcacaaactt tttttggaaa tgttggaggc caaggtctag
     1201 taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat atcttgtgga
     1261 aaggacgatg actccgacac gtctcaatgg ct
//
