LOCUS       sequence-281078-        1616 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2710
FEATURES             Location/Qualifiers
     source          1..1616
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggagcggtc acagtgtggc agcagagacc gaggcgttag cggccgacct
       61 cacttggccc ctgggctagt ggtggctgcc cctccgcccc cgtccccggc gttgccggta
      121 ccatcgggga tgcaggttcc cccagcgttc ctgcggccgc ccagcctctt tctgcgagca
      181 gcggccgcgg ccgccgccgc cgctgccgcc acctcgggaa gcggaggctg cccgccggct
      241 cccgggctgg agagcggggt aggcgcggtg ggctgcggct acccgcggac gcccaagtgc
      301 gcccgctgtc gtaaccatgg tgtggtgtca gcgctcaagg gccacaagcg cttctgccgc
      361 tggcgggact gcgcgtgtgc caagtgcacc ctgatcgccg agcgccagcg cgtcatggcc
      421 gcccaggtgg cgctgcgcag gcagcaggcg caggaggaga gcgaagcccg ggggctacag
      481 aggctcctgt gctcggggct ctcctggccc cccggtggtc gggcatccgg gggcggcggc
      541 agagccgaga atccacagtc cacgggcggc cctgcggcgg gggctgcgct gggactgggt
      601 gccttgagac aggccagtgg ttccgcgacc cccgctttcg aagttttcca gcaagattat
      661 cctgaggaaa aacaagaaca aaaagagagt aaatgtgagt catgccagaa tggacaagaa
      721 gaactgatct ccaaatccca tcagctttac ctaggatcat cttctaggtc taatggtgtc
      781 attgggaaac aaagtatcgg gtcatctatt tcagaatact ccaacaagcc tgatagtatc
      841 ctgtctcctc atcctggaga gcaatcagga ggtgaagaga gtcccaggtc cttatcatcc
      901 tctgatctgg aatcaggaaa tgaaagtgaa tgggtcaaag acttgactgc gaccaaggca
      961 agccttccga cagtgtcctc aagaccaaga gatcctcttg atatccttac taagattttc
     1021 ccaaattaca ggcgcagccg gctagaaggc attctacggt tctgcaaagg ggatgtggtc
     1081 caagccattg aacaggtttt aaatggcaaa gaacacaagc cagacaacag gaacctagca
     1141 aactcagaag aactggaaaa cacagccttt cagagagctt caagttttag tcttgctgga
     1201 attggttttg gaactctagg taataaatca gctttctctc ctcttcaaac tacttctgct
     1261 tcttatggag gtgattcaag tctctacggc gtaaatccta gagtaggtat cagtccatta
     1321 aggctggcat attcttctgc aggaagaggg ttatctggtt ttatgtcacc ctacctaaca
     1381 cctgggttag taccaacctt accttttcgg ccagctttgg attatgcctt ttcagggatg
     1441 attagagatt cttcctacct ttccagtaaa gactcaataa cttgtggcag actgtacttc
     1501 agaccaaatc aggacaatcc gtagtaatga actagtccgt aacttgaaag tatttcgatt
     1561 tcttggcttt atatatcttg tggaaaggac gatctcgcat ggtcccgctg ctttcg
//
