LOCUS       sequence-281084-        1496 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1496)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1496)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2731
FEATURES             Location/Qualifiers
     source          1..1496
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtgagtgg actgcgcctg gcttcccgaa gtggagaaga gggctggttg
       61 aaacctgctg tggcgcgact cgggcctcca cgccatcggc tccggaacct gcggaccgag
      121 tcgccgtggc gtagccgggg ctcagtcctc ttctgttcag ggccgggccg ggcggggcgg
      181 gcagcggaac ccctgcatcc tgtctgtacc tgcgggcgcc acttccgccg gccggagccc
      241 tgtcgggaac ccctcgcctc ccctatccag gactcggtgg cttttgagga tgtggctgtg
      301 aactttaccc aggaggaatg ggctttgcta gattcttctc agaagaatct ctacagagaa
      361 gtgatgcagg aaacctgcag gaacctggct tctgtaggaa gccaatggaa agaccagaat
      421 attgaagatc acttcgaaaa acctgggaaa gatataagaa atcatatcgt acagagactg
      481 tgtgaaagta aagaagatgg tcagtatgga gaagttgtca gccaaattcc aaatcttgat
      541 ctgaacgaga acatttctac tggattaaaa ccatgtgaat gcagtatttg tggaaaagtc
      601 tttgtacgtc attccctcct taataggcat atcctagctc actcaggata caaaccatat
      661 ggagagaagc aatataaatg tgaacagtgt gggaaattct tcgtttctgt tccaggtgtt
      721 agaagacaca tgataatgca cagtggaaat ccagcttata aatgtacgat atgtgggaaa
      781 gctttttatt ttctcaattc agttgaaaga catcagagaa ctcacacagg agaaaaaccc
      841 tataaatgta aacaatgtgg taaagcattc actgtttccg gttcttgtct aatacatgaa
      901 cgaactcaca ctggagagaa accctacgaa tgtaaggaat gtgggaaaac attcagattt
      961 tcttgttctt ttaagacgca tgaaaggact cacactggag aaagacccta taaatgtacc
     1021 aaatgtgata aagccttcag ctgttccact tcccttcgtt accatggaag cattcatact
     1081 ggagagagac cctatgagtg taaacaatgt ggcaaagcct ttagtcgttt gagttccctt
     1141 tgtaaccata gaagtactca taccggagag aaaccctatg aatgtaaaca atgtgatcaa
     1201 gccttcagtc gcctcagttc ccttcacctc cacgaaagaa ttcatactgg agaaaaaccc
     1261 tatgaatgta agaaatgcgg taaagcctac actcgttcca gtcaccttac tcgccatgaa
     1321 agaagtcatg atatagaggc tgggtgtagt gactcagcct ataatcccag cactttggga
     1381 ggccaaggcg tgtggattgc ttagtaatga actagtccgt aacttgaaag tatttcgatt
     1441 tcttggcttt atatatcttg tggaaaggac gacgtcatac aatggcgttc cacgac
//
