LOCUS       sequence-281090-        3005 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2763
FEATURES             Location/Qualifiers
     source          1..3005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctgtcgc cctcgactct cagatcgacg cgcccctgga ggttgaggga
       61 tgcctaataa tgaaggtgga aaaggaccct gagtgggcat cagagcccat tctggaagga
      121 tcggatagct ctgagacctt ccgcaaatgc ttcaggcaat tctgttatga ggatgtgact
      181 ggaccccatg aagctttcag taaactctgg gaactttgct gccggtggct gaagccagaa
      241 atgcgttcca aggagcaaat acttgagctg ctggtgattg agcagtttct caccatttta
      301 cccgagaaga ttcaggcttg ggcacagaag cagtgtccgc aaagtggaga ggaagcggtg
      361 gccctggtag tgcatttgga gaaagagact ggaagactaa gacagcaggt cagcagtccc
      421 gtgcaccggg agaagcactc cccacttgga gcagcgtggg aggtggcaga cttccagcca
      481 gagcaggtgg agacccaacc cagggcggtg tctcgggagg aacctggaag cctccactca
      541 ggacaccagg aacagctgaa ccgaaagcga gaacgtcggc ccttacccaa gaatgctcgg
      601 ccttctccct gggttcctgc ccttgctgat gaatggaata ccctagatca ggaagtgaca
      661 accacacggc ttcctgctgg gtcccaggaa ccagtgaaag atgtccacgt ggccagaggc
      721 ttttcctaca gaaagagtgt gcatcagatt cctgcccaaa gggacctcta ccgggatttc
      781 aggaaggaga atgttgggaa cgtggtctcc ctgggaagtg cagtgtctac atctaacaag
      841 ataacccggt tggaacagag aaaggagcca tggactctag gtctgcattc ctctaacaag
      901 agaagtatcc tacgaagcaa ctacgtcaag gaaaagtcag ttcatgctat tcaggtccct
      961 gcaaggagtg caggaaaaac atggagagag cagcagcagt ggggtttaga agatgaaaag
     1021 atagcaggtg tgcattggag ctatgaggaa acaaagactt tcctggcaat tctcaaagag
     1081 tctcgctttt atgaaacact tcaggcctgt ccccgaaata gccaagtgta tggtgctgtg
     1141 gctgaatggt tgcgagaatg tggcttcctt agaaccccag aacagtgtcg aaccaagttc
     1201 aaaagtctcc agaaaagcta tcgaaaggtg agaaatggcc acatgctaga accctgcgcc
     1261 ttctttgagg acatggatgc tttgttgaac cctgcagccc gtgctccgtc cactgataaa
     1321 ccaaaggaga tgatacctgt ccccagactg aagagaattg ccatcagtgc taaggaacac
     1381 atcagcttgg tggaggagga ggaagctgca gaagattctg atgatgatga aataggcatc
     1441 gaatttatcc gcaagtctga aatccatggt gcccctgtct tgtttcagaa tctcagtggc
     1501 gtgcactggg gctatgaaga aaccaagact tttcttgata tcctccgtga gactcggttt
     1561 tatgaagcgc ttcaagcctg tcatcggaag agcaaattgt atggggctgt agctgaacag
     1621 cttcgagagt gcggcttcct ccggacacca gaacagtgcc gaaccaagtt caaaagcctt
     1681 cagaagagtt accgcaaggt gaaaaatggc cacgtgctag agtcctgcgc gttctacaag
     1741 gagatggatg ccctgattaa ctctcgggca tctgctcctt cccccagcac cccagaggaa
     1801 gtcccatcac cttcaaggca agaaagaggg ggtattgagg ttgaacccca ggaacctaca
     1861 ggctgggaac ctgaagagac ctcacaggag gcagtaatag aagactcttg cagtgagaga
     1921 atgagcgagg aggaaattgt gcaagagcca gagttccagg gacctccagg tctactgcag
     1981 agcccaaatg attttgaaat cggaagtagc atcaaggagg atccaacaca gatagtatat
     2041 aaggacatgg aacagcatag ggcattaata gaaaagtcta aaagagttgt ttcccagagt
     2101 accgacccca gcaaatatcg caaaagggaa tgcatctcag gaagacaatg ggaaaatctt
     2161 caaggaatta gacagggaaa gccgatgtct cagcctagag atttagggaa agccgttgtg
     2221 catcagaggc cttttgtggg gaagagaccc tacagacttc tcaaatatgg agaaagcttt
     2281 ggaaggagca ctcgtctgat gtgccggatg acccaccaca aggagaatcc ttacaagtgt
     2341 ggtgtctgtg ggaagtgctt tggtagaagc aggagcctga tcagacacca aagaatccac
     2401 acaggcgaaa aaccttttaa atgtcttgac tgtggaaaaa gctttaatga ctcctcaaat
     2461 tttggtgccc accagagaat ccacacagga gagaaaccct acagatgcgg agagtgtgga
     2521 aaatgcttta gtcagagctc tagtcttatt atacatcaga gaacgcacac cggtgagaag
     2581 ccctatcagt gtggagagtg tgggaaaagt ttcaccaaca gttctcattt cagcgcccac
     2641 cggagagttc acactgggga gaatccctac aaatgtgtgg actgtgaaaa aagtttcaat
     2701 aactgtacga gatttcgaga acatcggaga atacacactg gagagaagcc ctatggatgt
     2761 gcccagtgtg gcaaacgttt cagtaagagt tctgttctta ccaaacatcg ggaagttcat
     2821 gtgagagaaa agcctctgcc acaccctcca tctctgtatt gccctgagaa cccacataag
     2881 ggaaagactg atgaatttag gaaaactttt tagtaatgaa ctagtccgta acttgaaagt
     2941 atttcgattt cttggcttta tatatcttgt ggaaaggacg atctgaactg acgtagcaga
     3001 ggttc
//
