LOCUS       sequence-281114-        2681 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2681)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2796
FEATURES             Location/Qualifiers
     source          1..2681
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             322..345
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
ORIGIN
        1 accgccacca tgaaaatcgg cagtgggttc ctgagtggcg gcggaggtac cggcagtagc
       61 ggtggtagcg gctccggcgg cggtggtagt ggcggcggcg gcggcggcgg cagcagcggc
      121 aggagggcag agatggaacc cacctttccc caggccccgg ccgctgagcc cccccctccg
      181 ccagccccgg acatgacttt caagaaggag ccggcggcgt cagccgcggc cttcccctcg
      241 cagaggacct cctgggggtt cttgcagtct ttggttagca tcaaacagga gaaacccgcg
      301 gatcctgagg agcagcagtc ccaccaccac catcaccacc accactatgg ggggctgttc
      361 gctggagctg aagagaggtc tccaggccta ggaggcggtg aaggggggag tcacggcgtc
      421 atccaggacc tcagtattct ccaccagcat gtccagcagc aaccagccca gcaccaccgt
      481 gacgtattac tcagcagcag tagcaggact gatgaccacc atggcactga ggagccaaag
      541 caggacacta atgtcaaaaa ggcaaaaagg ccaaagccag aatctcaggg aatcaaagcc
      601 aagaggaagc caagtgcatc ttccaaacct tctttggttg gagatggaga aggtgccatc
      661 ctctccccaa gtcagaaacc tcatatctgt gatcactgta gtgctgcttt ccgaagctcc
      721 tatcacctgc ggagacatgt cctcattcat acaggagaaa gacctttcca gtgcagccag
      781 tgtagtatgg gtttcattca gaaataccta ctacagagac atgagaaaat tcatagtaga
      841 gagaagccat ttggatgtga tcagtgcagc atgaagttta ttcagaagta ccatatggag
      901 agacacaaga ggacacatag tggagaaaag ccatataagt gtgacacttg ccaacagtat
      961 ttttcaagga ctgatagatt gttgaagcac aggcgcacat gtggtgaagt catagttaaa
     1021 ggagccacta gtgcagaacc tgggtcatca aaccatacca atatgggtaa tctggctgtg
     1081 ttgtctcagg gaaatacaag ttcttcaagg agaaaaacaa agtcaaaaag catagctatt
     1141 gaaaataagg aacagaagac cggtaaaaca aatgaatcgc aaatttcaaa taatataaac
     1201 atgcagagtt actcagtaga aatgcctacc gtgtcttcca gtggaggcat aattggcact
     1261 ggaatagatg aactgcagaa gagggtgcca aaattgatct ttaagaaagg aagcagaaag
     1321 aatacagata aaaactacct taactttgtg tcaccattac cagacatagt aggacagaaa
     1381 tccttgtctg gaaaaccaag tggctcactt ggcatagtat caaataatag tgtggagacc
     1441 attggtcttc tccaaagtac aagtggcaaa caaggtcaga taagtagtaa ttatgatgat
     1501 gccatgcagt tttcaaagaa aagaagatat ttaccaactg ccagcagcaa cagtgccttt
     1561 tctataaacg taggacacat ggtctcccaa cagtctgtca ttcagtctgc aggtgtcagt
     1621 gttttggaca atgaggcacc attgtcactt attgactcct cagctctaaa tgctgaaatt
     1681 aaatcttgtc atgacaagtc tggaattcct gatgaggttt tacaaagtat tttggatcaa
     1741 tactccaaca aatcagaaag ccagaaagag gatcctttca atattgcaga accacgagtg
     1801 gatttacaca cctcaggaga acactcagaa ttggttcaag aagaaaattt gagcccaggc
     1861 acccaaacac cttcaaatga taaagcaagt atgttgcaag aatactccaa atacctccaa
     1921 caggcttttg aaaaatccac taatgcaagt tttactcttg gacacggttt ccaatttgtc
     1981 agtttgtctt cacctctcca caaccacact ttgtttccag aaaaacaaat atacactacg
     2041 tctcctttgg agtgtggttt cggccaatct gttacctcag tgttgccatc ttcattgcca
     2101 aagcctcctt ttgggatgtt gtttggatct cagccaggtc tttatttgtc tgctttggat
     2161 gctacacatc agcagttgac accttcccag gagctggatg atctgataga ttctcagaag
     2221 aacttagaga cttcatcagc cttccagtcc tcatctcaga aattgactag ccagaaggaa
     2281 cagaaaaact tagagtcttc aacaggcttt cagattccat ctcaggagtt agctagccag
     2341 atagatcctc agaaagacat agagcctaga acaacgtatc agattgagaa ctttgcacaa
     2401 gcgtttggtt ctcagtttaa gtcgggcagc agggtgccaa tgacctttat cactaactct
     2461 aatggagaag tggaccatag agtaaggact tcagtgtcag atttctcagg gtatacaaat
     2521 atgatgtctg atgtaagtga gccatgtagt acaagagtaa agacacccac cagccagagt
     2581 tacaggtagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     2641 tcttgtggaa aggacgaatg tacatcttac cagatctccc t
//
