LOCUS       sequence-28114-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peCALWY-1
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(555..576)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttgacctgcc caacaagaag gnctgcattg aatctgagca cagcatggac actctgcttg
       61 caaccctgaa gaaaacagga aagactgttt cctaccttgg ccttgagctc attcgtggcg
      121 gatccggcgg aagcggcgga tccggcggta gcggcggatc cggcggctcc ggcggatccg
      181 gcggcagcgg cggatccggt ggaagcggtg gatccggtgg tagcggtgga tccggtggaa
      241 gcggtggatc cggtggtagc ggtggatccg ggggtccgcg gatgcanggc acatgcagta
      301 ccactctgat tgccattgcc ggcatgacct gtgcatcctg tgtccattcc attgaaggca
      361 tgatctccca actggaaggg gtgcagcaaa tatcggtgtc tttggccgaa gggactgcaa
      421 cagttcttta taatcccgct gtaattagcc cagaanaact cagagctgct atagaagaca
      481 tgggatttga ggcttcagtc tcggtaccca tggtgagcaa gggcgaggag ctgttcaccg
      541 gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggncacnag ttcagcgtgt
      601 ccggcgaggg cgagggcgat gccacctacn gcaagctgac cctgaagttc atctgcacca
      661 ccggcaagct gcccgtgccc tggcncaccc tcgtgaccac cttcngctac ggnctgatgt
      721 gcttcgcccg ctaccccgac cacatgaagc a
//
