LOCUS       sequence-281150-        1529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2832
FEATURES             Location/Qualifiers
     source          1..1529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggaagatat acaaacaaat gcggaactga aaagcactca ggagcagtct
       61 gtgcccgcag atgattcaat gaaagtgaaa gatgaataca gtgaaagaga tgagaatgtt
      121 ttaaagtcag aacccatggg aaatgcagaa gagcctgaaa tcccttacag ctattcaaga
      181 gaatataatg aatatgaaaa cattaagttg gagagacatg ttgtctcatt cgatagtagc
      241 aggccaacca gtggaaagat gaactgcgat gtgtgtggat tatcctgcat cagcttcaat
      301 gtcttaatgg ttcataagcg aagccatact ggtgaacgcc cattccagtg taatcagtgt
      361 ggggcatctt ttactcagaa aggtaacctc ctccgccaca ttaaactgca cacaggggaa
      421 aaacctttta agtgtcacct ctgcaactat gcatgccaaa gaagagatgc gctcacgggg
      481 catcttagga cacattctgt ggagaaaccc tacaaatgtg agttttgtgg aaggagttac
      541 aagcagagaa gttcccttga ggagcacaag gagcgctgcc gtacatttct tcagagcact
      601 gacccagggg acactgcaag tgcggaggca agacacatca aagcagagat gggaagtgaa
      661 agagctctcg tactggacag attagcaagc aatgtggcaa aacgaaaaag ctcaatgcct
      721 cagaaattca ttggtgagaa gcgccactgc tttgatgtca actataattc aagttacatg
      781 tatgagaaag agagtgagct catacagacc cgcatgatgg accaagccat caataacgcc
      841 atcagctatc ttggcgccga agccctgcgc cccttggtcc agacaccgcc tgctcccacc
      901 tcggagatgg ttccagttat cagcagcatg tatcccatag ccctcacccg ggctgagatg
      961 tcaaacggtg cccctcaaga gctggaaaag aaaagcatcc accttccaga gaagagcgtg
     1021 ccttctgaga gaggcctctc tcccaacaat agtggccacg actccacgga cactgacagc
     1081 aaccatgaag aacgccagaa tcacatctat cagcaaaatc acatggtcct gtctcgggcc
     1141 cgcaatggga tgccacttct gaaggaggtt ccccgctctt acgaactcct caagcccccg
     1201 cccatctgcc caagagactc cgtcaaagtg atcaacaagg aaggggaggt gatggatgtg
     1261 tatcggtgtg accactgccg cgtcctcttc ctggactatg tgatgttcac gattcacatg
     1321 ggctgccacg gcttccgtga ccctttcgag tgtaacatgt gtggatatcg aagccatgat
     1381 cggtatgagt tctcgtctca catagccaga ggagaacaca gagccctgct gaagtagtaa
     1441 tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag
     1501 gacgaggtta tcagaaccgt actatcgtc
//
