LOCUS       sequence-281156-        1847 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1847)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0043
FEATURES             Location/Qualifiers
     source          1..1847
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggccatgtc ccaggaatca ttgaccttca aggacgtgtt tgtggacttc
       61 accctggagg agtggcagca actggactct gcccagaaga acctctacag ggatgtcatg
      121 cttgagaact acagccacct ggtgtccgtg gggcatctag ttgggaagcc agatgtgatc
      181 ttcaggttgg gaccaggtga cgagtcctgg atggcagatg gagggacccc ggtacggacc
      241 tgtgcaggtg aggacaggcc agaagtctgg gaagttgacg agcagataga tcactacaag
      301 gaaagccaag acaaatttct gtggcaagct gcattcatag gcaaggaaac actgaaggat
      361 gaaagtggtc aagaatgtaa aatatgtaga aaaatcattt atctcaacac agactttgtt
      421 tctgtaaaac aaagactccc taaatattat tcatgggaaa ggtgttcaaa acatcattta
      481 aactttcttg gtcaaaatag aagctatgta agaaagaaag atgatggatg taaggcatat
      541 tggaaagtat gcctccatta taatcttcat aaagctcaac ctgcagagag attttttgac
      601 cctaatcaac gagggaaagc cctccaccaa aagcaagccc ttagaaaaag tcagagaagt
      661 caaactgggg agaaactcta caaatgtact gaatgtggaa aagtgtttat ccagaaagca
      721 aacttagttg tacatcaaag aactcacacc ggagagaaac cttatgaatg ctgcgaatgt
      781 gcaaaagcct tcagccagaa gtcaaccctc atagcacacc agagaactca cacaggggag
      841 aagccctatg aatgcagtga atgtggaaaa acctttatcc agaagtcaac tctgattaaa
      901 catcaacgaa ctcatacagg agagaaacca tttgtatgtg acaaatgtcc aaaagccttt
      961 aagagttcat atcatcttat tagacatgaa aaaacacaca ttagacaagc attttataaa
     1021 ggtattaaat gtactacgtc cagccttata tatcaaagaa ttcacacaag tgagaaacct
     1081 cagtgcagtg aacatgggaa agcctctgat gagaagccca gtcccactaa acattggaga
     1141 actcatacaa aagagaacat ttatgagtgt agtaaatgtg ggaaaagctt cagaggaaag
     1201 tcacacctct ctgttcatca gagaattcat acaggagaga aaccctatga atgtagtata
     1261 tgtgggaaga ctttcagtgg aaagtcacac ctctctgtcc atcatagaac tcatacagga
     1321 gagaaacctt atgaatgcag aagatgtggg aaagcctttg gggagaagtc aacccttatt
     1381 gtacatcaga gaatgcatac aggagagaaa ccctataaat gcaacgaatg tgggaaagcc
     1441 tttagtgaga agtcaccact gattaaacat cagagaattc atacaggaga gagaccctat
     1501 gaatgcactg actgtaaaaa agccttcagt aggaagtcaa ctctcatcaa acatcagaga
     1561 attcatacag gagaaaaacc ttacaaatgt agtgaatgtg ggaaggcctt cagtgtgaaa
     1621 tcaactctca ttgtgcatca cagaactcat acaggagaga aaccttatga atgcagagac
     1681 tgtgggaaag cattcagtgg gaagtcaact ctcattaaac atcagagaag tcacacagga
     1741 gataaaaacc tatagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1801 tatatatctt gtggaaagga cgattacgca taaataagcc cgcaaga
//
