LOCUS       sequence-281159-        1313 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1313)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0058
FEATURES             Location/Qualifiers
     source          1..1313
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             553..570
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 accgccacca tggcatcaga actggcaatg agcaactccg acctgcccac cagtcccctg
       61 gccatggaat atgttaatga cttcgatctg atgaagtttg aagtgaaaaa ggaaccggtg
      121 gagaccgacc gcatcatcag ccagtgcggc cgtctcatcg ccgggggctc gctgtcctcc
      181 acccccatga gcacgccgtg cagctcggtg cccccttccc ccagcttctc ggcgcccagc
      241 ccgggctcgg gcagcgagca gaaggcgcac ctggaagact actactggat gaccggctac
      301 ccgcagcagc tgaaccccga ggcgctgggc ttcagccccg aggacgcggt cgaggcgctc
      361 atcagcaaca gccaccagct ccagggcggc ttcgatggct acgcgcgcgg ggcgcagcag
      421 ctggccgcgg cggccggggc cggtgccggc gcctccttgg gcggcagcgg cgaggagatg
      481 ggccccgccg ccgccgtggt gtccgccgtg atcgccgcgg ccgccgcgca gagcggcgcg
      541 ggcccgcact accaccacca ccaccaccac gccgccggcc accaccacca cccgacggcc
      601 ggcgcgcccg gcgccgcggg cagcgcggcc gcctcggccg gtggcgctgg gggcgcgggc
      661 ggcggtggcc cggccagcgc tgggggcggc ggcggcggcg gcggcggcgg aggcggcggg
      721 ggcgcggcgg gggcgggggg cgccctgcac ccgcaccacg ccgccggcgg cctgcacttc
      781 gacgaccgct tctccgacga gcagctggtg accatgtctg tgcgcgagct gaaccggcag
      841 ctgcgcgggg tcagcaagga ggaggtgatc cggctgaagc agaagaggcg gaccctgaaa
      901 aaccgcggct atgcccagtc ctgccgcttc aagagggtgc agcagagaca cgtcctggag
      961 tcggagaaga accagctgct gcagcaagtc gaccacctca agcaggagat ctccaggctg
     1021 gtgcgcgaga gggacgcgta caaggagaaa tacgagaagt tggtgagcag cggcttccga
     1081 gaaaacggct cgagcagcga caacccgtcc tctcccgagt ttttcataac tgagcccact
     1141 cgcaagttgg agccatcagt gggatacgcc acattttgga agccccagca tcgtgtactt
     1201 accagtgtgt tcacaaaata gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1261 tggctttata tatcttgtgg aaaggacgag taccaactat ctaagcaggg tga
//
