LOCUS       sequence-28116-e         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peCALWY-2
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(483..504)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttgacctgcc cnacaagaag gtctgcattg aatctgagca cagcatggac actctgcttg
       61 caaccctgaa gaaaacagga aagactgttt cctaccttgg ccttgagctc attcgtggcg
      121 gatccggcgg aagcggcgga tccggcggta gcggcggatc cggcggctcc ggcggatccg
      181 gtggtagcgg tggatccggg ggtccgcgga tgcagggcac atgcagtacc actctgattg
      241 ccattgccgg catgacctgt gcatcctgtg tccattccat tgaaggcatg atctcccaac
      301 tggaaggggt gcagcaaata tcggtgtctt tggccgaagg gactgcaaca gttctttata
      361 atcccgctgt aattagccca gaagaactca gagctgctat agaagacatg ggatttgagg
      421 cttcagtctc ggtacccatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca
      481 tcctggtcga gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg
      541 agggcgatgc cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc
      601 ccgtgccctg gcccaccctc gtgaccacct tcggctacgg cctgatgtgc ttcgcccgct
      661 accccgacca catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc
      721 aggagcgcac catcttcttc aaggacgacg gcaactacna ganccgcgcc gangtgaagt
      781 tcgagggcga caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg
      841 gcancatcct ggggcacaag cttgagtaca actacaacag ccacaacgtc tatatcatg
//
