LOCUS       sequence-281162-        1781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0066
FEATURES             Location/Qualifiers
     source          1..1781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggagaccc ggggtcggag ataatagaat ctgtccctcc agctggccct
       61 gaggcatctg agtcaacaac ggatgaaaat gaagacgaca ttcagtttgt cagtgaagga
      121 ccattacgac ctgttcttga atacattgat ctggtcagca gtgatgatga agagcctagc
      181 acctcttata ctgatagaat gcctgaatct aaggtgccat cctctgagaa tcatcgccca
      241 gaaatgtgct ctagctgcaa tgttcctctt cccattggag atagcagctc cttctctggg
      301 agttgttcca gcagtccaga aaggatagtt tctcaaactt cctctgttga gaacccattg
      361 gagaaccaga aaaatgatca aaataattca gatactaaga tctctgagac agagaccctt
      421 aaatcatcac agaattttca gactctgcct tcatctccac ttctggtccc ccaagaatct
      481 ttggcctctt ctgaggtcaa agagaattta cgtatagatt cttcttcagc ttcacagcat
      541 ggacgggatg ccatcctcta tctccagaca caagtagctg aaatgtcccg agtgatacgt
      601 gatctgcagt ccaggagctg ttttagattt catcattcta ggccaagtga gaactcctca
      661 gttccttggg acatctccac ctctaaagag gaaaatttat ccacagttga agaagaaact
      721 gattacaaat caccatcagc tgatgacaaa gggcagccat ctgaccccag ccaatctagt
      781 ttcacaggtc ttttgaagag aatggaacaa agaggtgtta taaaaagggt gacattacaa
      841 tctgaagcgg agtcatgtga agggaaacct gattgtgtga cttctaaaaa acgtttggtt
      901 cctccattgc atcctcttct gagaattgcc accactgagg tttttaaaga ccctgctgat
      961 tgccatcctt cttccttcat gggacacagg gtatatcctg tggccaagga cacctctcct
     1021 ttccaaccaa acccaccagc tgaaggcccc attgtagaag cattagaaca cagcaaaaga
     1081 ggaaacacaa catcccctct agattctacc tcaaaagaaa tggaggtcat gggttgtagg
     1141 ttttaccacg ctgcctccat tgcagcccga gctgctagct acatggccta tatgactcaa
     1201 tatcagcgta aactctggga agacatggaa gatctggttc atgacccaga atttgatcgt
     1261 ggaaaagcaa gatgcataat atctgatggt atggatgcag gcctttggca actttgtact
     1321 actagggaca taatggactc tgtagtcaga gttatggcca tggccataga ctatagacgg
     1381 caggcctggc ttcgacttac atctctcact aagaaaaccc aggagaagat ctcccacttg
     1441 ccctttgatg gtacttccct ttttggacaa gatgtgaaag ctgttgttgc agaagacaac
     1501 aatataaaag aaaatgacta taaagatcac aaatactata atcagcatcg atacttttat
     1561 agtcatgatc agaaagcaca ttatcacaat agaggatact ccaaagggga ttggtacaaa
     1621 cctcgaaacc acccctatag atatagaaag aagggagact ctccagaacg ccatgggtac
     1681 aagaattagt aatgaactag tccgtaactt gaaagtattt cgatttcttg gctttatata
     1741 tcttgtggaa aggacgatgt aggccgaacc tcgtaagccc g
//
