LOCUS       sequence-281171-        3455 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0108
FEATURES             Location/Qualifiers
     source          1..3455
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tgggtgacca gcaactgtac aagaccaacc atgtggccca tggtagtgag
       61 aaccttttct accaacagcc accacttggc gtccacagcg ggctgaacca caactatggg
      121 aatgcagtta cagggggcgg aatggatgcc cctcaggcct cgccaatctc cccccacttc
      181 cctcaagata cacgggatgg tctgggcttg cctgttggct ccaaaaacct tggccaaatg
      241 gatacctcga ggcagggagg gtggggaagt catgcagggc ctggaaacca tgtccagcta
      301 cgtggaaacc tggccaactc aaacatgatg tggggggcac cagcccaggc tgagcccact
      361 gatggctacc aatacaccta ctcccaggcc agcgagatcc ggacccagaa gcttaccagc
      421 ggtgtcttac acaagctgga ctctttcacc caggtgtttg ccaaccaaaa cctgcgaatt
      481 caggtcaaca atatggccca ggtgctgcac actcagtcag cagtgatgga tggagcccct
      541 gacagtgctc tccgccagct gctgtctcag aagcccatgg agcccccagc accggctatc
      601 ccttcccgct accagcaggt gccccagcag cctcaccctg gtttcactgg tgggctgtcc
      661 aaaccagctc ttcaggtcgg gcagcaccct acccaagggc acctgtatta tgactaccag
      721 cagcctctgg ctcaggtgcc agtgcaggga ggacagccac tgcaggcccc acagatgctg
      781 tcacagcaca tgcaacagat gcagcagcac cagtattacc caccgcagca acagcagcaa
      841 gccgggcaac agcgtatctc catgcaagaa atacagacgc agccgcaaca aattcgccca
      901 tcacagccac agccgccgcc acagcagcag cagccgcagc agctacagct gcagcagcgg
      961 cagggttcaa tgcagatacc tcagtattat cagccccaac ccatgatgca gcacttgcaa
     1021 gagcagcagc agcaacagat gcacctgcag cctccttctt atcacaggga ccctcaccag
     1081 tataccccag agcaggcaca cactgtccag ctgattcccc tgggctccat gtcccagtac
     1141 tactaccagg agccccagca gccctacagc caccccctct accagcagag ccacctgtcc
     1201 cagcaccagc agcgtgagga cagtcagctg aagacctact ctagtgacag acaggcccag
     1261 gccatgctga gctcccatgg ggacctgggg cctcctgaca caggaatggg agacccagcg
     1321 agctcagatc tgacccgggt cagcagcacc ctcccccatc gccccctcct atcccccagt
     1381 gggatccacc tcaacaacat ggggcctcag catcagcagc tgtctcccag tgccatgtgg
     1441 ccccagatgc acctacctga tgggagagcc cagccagggt cccctgagtc aagtggccaa
     1501 cccaaaggag cgtttgggga gcagtttgat gccaagaaca agctgacatg ctccatctgc
     1561 ctgaaggagt tcaagaacct gcctgccctg aatggccaca tgcggtccca cgggggaatg
     1621 agggcctccc ccaacctcaa acaggaaatc cccaggaagc atcagccgag tgtgcccaaa
     1681 gccgaggagc ccctcaagac cgtgcaggag aagaaaaagt tccggcaccg gccggaacct
     1741 ctcttcatcc cgccgccgcc ctcctacaac ccgaaccccg ctgcctccta ctcgggcgcc
     1801 accctgtacc agagccagct gcgctccccg cgcgtcctcg gggaccacct gctcctggac
     1861 cccacccacg agctgccccc ttacacgccc ccacccatgc tgagcccggt gcgccagggc
     1921 tcggggctct tcagcaatgt cctcatctcc ggccacggcc ctggcgccca cccgcagctg
     1981 cccctgacgc ccctgacgcc cacaccacgg gtgctgctgt gtcgctccaa cagcatcgat
     2041 ggcagcaacg tgacggtcac cccagggcct ggagagcaga ctgtagatgt tgaaccacgc
     2101 atcaacattg gcttgagatt ccaagcagaa atccctgaac tccaagatat ctctgccctg
     2161 gcccaggaca cacacaaggc cacactggta tggaagccct ggccagaact agaaaaccat
     2221 gacctccagc aaagagtgga gaatcttctg aatttgtgct gttccagtgc attgccaggt
     2281 ggagggacca attctgaatt tgctttgcac tctctgtttg aggccaaagg tgatgtgatg
     2341 gttgctctgg aaatgctgct actgcggaag cctgtcaggt taaaatgtca tcctttagca
     2401 aattaccact atgccggttc ggacaagtgg acctccctag aaagaaaact gtttaacaaa
     2461 gcactagcca cttacagcaa agactttatt tttgtacaga agatggtgaa gtccaagacg
     2521 gtggctcagt gcgtggagta ctactacacg tggaaaaaga tcatgcggct ggggcggaaa
     2581 caccggacac gcctggcaga aatcatcgac gattgtgtga caagtgaaga agaagaagag
     2641 ttagaggagg aggaggagga ggacccggaa gaagatagga aatccacaaa agaagaagag
     2701 agtgaggtgc cgaagtcccc ggagccacca cccgtccccg tcctggctcc cacggagggg
     2761 ccgcccctgc aggccctggg ccagccctca ggctccttca tctgtgaaat gcccaactgt
     2821 ggggctgtgt tcagctcccg acaggcactg aatggccatg cccgcatcca cgggggcacc
     2881 aaccaggtga ccaaggcccg aggtgccatc ccctctggga agcagaagcc tggtggcacc
     2941 cagagtgggt actgttcggt aaagagctca ccctctcaca gcaccaccag cggcgagaca
     3001 gaccccacca ccatcttccc ctgcaaggag tgtggcaaag tcttcttcaa gatcaaaagc
     3061 cgaaatgcac acatgaaaac tcacaggcag caggaggaac aacagaggca aaaggctcag
     3121 aaggcggctt ttgcagctga gatggcagcc acgattgaga ggactacggg gcccgtgggg
     3181 gcgccggggc tgctgcccct ggaccagctg agtctgatca aacccatcaa ggatgtggac
     3241 atcctcgacg acgacgtcgt ccagcagttg ggaggtgtca tggaagaggc tgaagttgtg
     3301 gacaccgatc ttctcttgga tgatcaagat tcagtcttgc ttcagggtga cgcagaacta
     3361 tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta tatatcttgt
     3421 ggaaaggacg agatctatct tccgtggttc ttcag
//
