LOCUS       sequence-281177-         704 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 704)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 704)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0130
FEATURES             Location/Qualifiers
     source          1..704
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggctaaagg tgaccccaag aaaccaaagg gcaagatgtc cgcttatgcc
       61 ttctttgtgc agacatgcag agaagaacat aagaagaaaa acccagaggt ccctgtcaat
      121 tttgcggaat tttccaagaa gtgctctgag aggtggaaga cgatgtccgg gaaagagaaa
      181 tctaaatttg atgaaatggc aaaggcagat aaagtgcgct atgatcggga aatgaaggat
      241 tatggaccag ctaagggagg caagaagaag aaggatccta atgctcccaa aaggccaccg
      301 tctggattct tcctgttctg ttcagaattc cgccccaaga tcaaatccac aaaccccggc
      361 atctctattg gagacgtggc aaaaaagctg ggtgagatgt ggaataattt aaatgacagt
      421 gaaaagcagc cttacatcac taaggcggca aagctgaagg agaagtatga gaaggatgtt
      481 gctgactata agtcgaaagg aaagtttgat ggtgcaaagg gtcctgctaa agttgcccgg
      541 aaaaaggtgg aagaggaaga tgaagaagag gaggaggaag aagaggagga ggaggaggag
      601 gaggatgaat agtaatgaac tagtccgtaa cttgaaagta tttcgatttc ttggctttat
      661 atatcttgtg gaaaggacga tttcacaaat cgctcccgag tcct
//
