LOCUS       sequence-281180-        2027 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0135
FEATURES             Location/Qualifiers
     source          1..2027
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggtccatgg atcagtgaca ttcagggatg tggccattga cttctctcag
       61 gaggagtggg agtgcctgca gcctgatcag aggaccttgt acagggatgt gatgttggag
      121 aactacagcc acctgatctc actgggaagt tccatttcta aaccagatgt aattacgtta
      181 ctagagcaag agaaagagcc ctggatggtt gtaaggaaag aaacaagcag acggtatcca
      241 gatttggagt taaaatatgg acctgagaaa gtatctccag aaaatgatac ctctgaagta
      301 aatttaccca aacaggttat aaagcaaata agtacaactc ttggcattga ggccttttat
      361 tttagaaatg actcagaata tagacaattt gagggactac agggatatca agaaggaaat
      421 atcaatcaaa agatgatcag ctatgaaaaa ctgcctactc atactcctca tgcttctctt
      481 atttgcaata cacataaacc gtatgaatgt aaggaatgtg ggaaatactt tagtcgtagt
      541 gcaaatctta ttcagcatca gagtattcat actggagaga aaccctttga atgtaaggag
      601 tgtgggaaag cctttcgact tcacatacaa tttactcgac atcagaaatt tcatactggt
      661 gagaaacctt ttgaatgtaa cgaatgtgga aaggccttta gtcttcttac cctgcttaat
      721 cgccataaga acattcacac aggtgagaaa ctgtttgaat gtaaggaatg tgggaagtcc
      781 tttaatcgta gctcaaacct tgttcaacat cagagtattc attctggtgt aaaaccatat
      841 gaatgtaagg agtgtgggaa aggctttaat cgtggtgcac accttattca gcatcagaaa
      901 attcattcca atgagaaacc ctttgtatgt aaggaatgtg ggatggcctt tcgatatcat
      961 taccaactta ttgaacattg ccaaattcat actggtgaga aaccctttga atgtaaagaa
     1021 tgtggaaagg cgtttactct tctgacaaag cttgttcgac atcagaagat tcatactggt
     1081 gagaaaccct ttgaatgcag ggaatgtggg aaggccttta gtcttctcaa ccagcttaat
     1141 cgccataaga acattcacac aggtgaaaaa ccgtttgaat gtaaggaatg tgggaagtcc
     1201 tttaatcgta gctcaaacct tgttcaacat cagagtattc atgctggtat aaaaccatat
     1261 gaatgtaagg agtgtgggaa aggctttaat cgtggtgcac accttattca gcatcagaaa
     1321 attcattcca atgagaaacc ttttgtatgt agggaatgtg agatggcctt tagatatcat
     1381 tgccaactta ttgaacattc tcgaattcat actggtgaca agccatttga atgtcaagac
     1441 tgtgggaagg ccttcaatcg tggctcaagc cttgttcaac atcagagtat tcacactggt
     1501 gagaagccct atgaatgtaa ggagtgtggg aaggctttta gactttacct acaactttcc
     1561 caacatcaga aaactcacac aggtgaaaaa ccatttgaat gtaaggaatg tgggaaattc
     1621 tttcgtcgtg gttcaaatct taatcaacat cgaagtattc atactggaaa gaaacccttt
     1681 gaatgtaagg aatgtgggaa agcctttcga cttcatatgc accttattcg acatcagaaa
     1741 ttgcatactg gtgagaaacc ctttgaatgt aaggagtgtg ggaaagcctt tcgacttcat
     1801 atgcaactta ttcgacatca gaaattgcat actggtgaga aaccctttga atgtaaggaa
     1861 tgtggaaagg tttttagtct tcccacccag cttaatcgcc ataagaacat tcacacaggt
     1921 gagaaggcat cttagtaatg aactagtccg taacttgaaa gtatttcgat ttcttggctt
     1981 tatatatctt gtggaaagga cgaccgcacc gggtgaatac tggaaca
//
