LOCUS       sequence-28119-e         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peCALWY-4
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      34..43
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(49..51,52..54,55..765)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALFKDPNEKRDHMVLL
                     EFLTAAGITLGMDELYK"
     primer_bind     complement(94..115)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(355..374)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctggtttagt gnaccgtcag atccgctagc gccgccacca tgggccatat ggtgagcaag
       61 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      121 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      181 ctgaagttca tctgcaccac cggtaagctg cccgtgccct ggcccaccct cgtgaccacc
      241 ctgacctggg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      301 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      421 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      481 aacgccatca gcgacaacgt ctatatcacc gccgacaagc agaagaacgg catcaaggcc
      541 aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag
      601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      661 cagtccgccc tgttcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      721 ctgaccgccg ccgggatcac tctcggcatg gacgagctgt acaag
//
