LOCUS       sequence-281192-        1493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0334
FEATURES             Location/Qualifiers
     source          1..1493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcccaggg tttggtgacg ttcgccgacg tagccataga cttttctcag
       61 gaggagtggg cctgtctgaa ctctgctcag agggacctgt actgggacgt gatgctggag
      121 aactacagta acttggtctc actggatttg gagtcagcat atgaaaataa gagtttacct
      181 acagaaaaaa acattcatga aataagggct tccaaaagga attcagatag aagaagtaaa
      241 tcccttggcc gtaactggat atgtgaaggt acgcttgaaa gaccacagcg ctccagaggg
      301 aggtatgtca atcagatgat catcaattat gtcaaaagac ctgctactag agaaggcacc
      361 cctcctagaa cacatcagag acatcataag gagaattcct ttgaatgtaa ggactgtggg
      421 aaggccttta gtcgtggcta tcaacttagt caacatcaga aaatccatac tggtgagaaa
      481 ccttatgaat gtaaagaatg taagaaggcc ttccgttggg gcaatcagct tactcaacat
      541 caaaaaattc atactgggga gaagccctac gaatgtaaag actgtgggaa ggcttttcga
      601 tggggctcaa gcctcgttat tcataagagg attcatactg gtgaaaaacc ctatgaatgt
      661 aaagactgtg gaaaggcctt tcggcgtggt gatgagctca ctcagcacca gagattccac
      721 actggggaga aagactacga atgcaaagac tgtgggaaga cctttagccg tgtgtataaa
      781 cttattcagc acaagagaat tcatagtggg gagaagcctt acgagtgtaa agactgtggg
      841 aaggctttta tttgtggttc aagcctcatt cagcataaaa gaattcacac aggtgagaaa
      901 ccctatgaat gtcaagaatg tgggaaggcc tttactcgag tcaattacct tactcagcat
      961 cagaagatcc acaccggtga gaagcctcac gaatgtaagg agtgtgggaa ggcctttcgc
     1021 tggggttcga gcctcgttaa gcacgagagg atacatacgg gcgagaagcc gtacaagtgc
     1081 acagaatgtg ggaaggcctt caattgtggc tatcacctca ctcagcacga gagaatccac
     1141 acaggcgaaa ccccgtataa atgtaaggag tgtgggaagg ctttcattta tggatcgagc
     1201 ctcgtgaaac atgagagaat tcataccggg gtgaaaccct atgggtgtac agaatgtggg
     1261 aagagcttta gtcacggcca tcagcttaca caacatcaga aaacgcacag tggggcgaaa
     1321 tcctacgaat gtaaggagtg cgggaaggca tgtaaccacc taaaccatct ccgagaacat
     1381 cagaggatcc acaacagtta gtaatgaact agtccgtaac ttgaaagtat ttcgatttct
     1441 tggctttata tatcttgtgg aaaggacgac cagggctccc atgtatggtt ggt
//
