LOCUS       sequence-28120-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Vinkenborg JL, Nicolson TJ, Bellomo EA, Koay MS, Rutter GA, Merkx M
  TITLE     Genetically encoded FRET sensors to monitor intracellular Zn(2+) 
            homeostasis.
  JOURNAL   Nat Methods. 2009 Oct;6(10):737-40. doi: 10.1038/nmeth.1368.
  PUBMED    19718032
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2009-10"; volume: "6"; issue: 
            "10"; pages: "737-40"
COMMENT     Sequence Label: peCALWY-5
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(494..515)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ttagtatgac nttgacctgc ccaacaagaa ggnctgcatt gaatctgagc acagcatgga
       61 cactctgctt gcaaccctga agaaaacagg aaagactgtt tcctaccttg gccttgagct
      121 cattcgtggc ggatccggcg gaagcggcgg atccggcggt agcggcggat ccggcggctc
      181 cggcggatcc ggcggcagcg gcggatccgg gggtccgcgg atgcagggca catgcagtac
      241 cactctgatt gccattgccg gcatgacctg tgcatccagt gtccattcca ttgaaggcat
      301 gatctcccaa ctggaagggg tgcagcaaat atcggtgtct ttggccgaag ggactgcaac
      361 agttctttat aatcccgctg taattagccc agaagaactc agagctgcta tagaagacat
      421 gggatttgag gcttcagtct cggtacccat ggtgagcaag ggcgaggagc tgttcaccgg
      481 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
      541 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
      601 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgatgtg
      661 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      721 angctacgtc caggagcgca ccatcttctt caaggacgac ggcanctaca agacccgcgc
      781 cgangtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
      841 caaggaggac ggcaacatcc tggggcacaa gcttgagtac aactacaaca gccacaacgt
      901 ctat
//
