LOCUS       sequence-281210-        1322 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1322)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 1322)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0436
FEATURES             Location/Qualifiers
     source          1..1322
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      4..13
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 accgccacca tggcttcaac agaggaacag tacgatctta agattgtgaa agtggaggaa
       61 gaccctatct gggaccaaga aacccacctt cgagggaaca acttttctgg ccaagaagcc
      121 tcccgacaac tttttaggca gttttgttac caagagactc ctggtccccg agaagctctg
      181 agccggctcc gagaactctg tcatcagtgg ctaaggccag aaatccacac caaagagcaa
      241 atcttggagc tgctggtgct ggagcaattc ctgactatcc tgcctgagga gctccaggcc
      301 tgggtgcggg agcaccatcc ggagagtggg gaggaggctg tggctgtagt tgaagatctg
      361 gaacaagagc ttagtgagcc agggaaccag gctccagacc atgaacatgg acattctgaa
      421 gtgctcttgg aggatgtgga acatctgaag gtcaagcagg aaccaacaga catacagctt
      481 cagcctatgg tgacacagct cagatatgaa tctttttgcc tccaccaatt tcaagaacaa
      541 gatggtgaaa gtatacctga gaaccaggag ttggcatcaa agcaagaaat cttaaaagaa
      601 atggaacatt tgggggatag caaactccaa agagatgtat ctttggattc taagtacaga
      661 gaaacttgta aacgagacag caaggcagaa aagcagcagg cacattccac tggagagaga
      721 cgccacaggt gcaatgaatg tgggaaaagc ttcactaaga gttcagtact cattgagcac
      781 cagagaatcc acactgggga gaagccatat gaatgtgaag aatgtgggaa ggccttcagc
      841 cggaggtcaa gcctgaatga acatcggcgg agccacactg gagagaaacc ctatcaatgt
      901 aaggagtgtg ggaaagcctt cagtgccagc aatggcctca ctcgacacag aagaatccac
      961 acaggggaaa aaccatatga atgcaaagtg tgtgggaagg ctttcctcct cagctcatgc
     1021 cttgttcagc atcagaggat acacactgga gagaagcgct atcagtgtcg tgagtgtggc
     1081 aaagccttca ttcagaatgc agggcttttc cagcatctcc gagtccacac tggtgagaaa
     1141 ccctatcagt gcagtcagtg cagtaaactc tttagtaagc ggacacttct taagaaacat
     1201 cagaaaatcc acactggaga gagaccatag taatgaacta gtccgtaact tgaaagtatt
     1261 tcgatttctt ggctttatat atcttgtgga aaggacgaat acaatacagg attctgtccg
     1321 ta
//
